ProtParam
User-provided sequence:
10 20 30 40 50 60
MSGNQMAMGS EQQQTVGSRT VSVEEVPAVL QLRATQDPPR SQEAMPTRHN VRWEENVIDN
70 80 90 100 110 120
ENMNKKKTKI CCIFHPQNED EEECNHHSDD DGSSSSGSSS SESENEKDLD FNERRQRRLE
130 140 150
RRHRKLEKKR SYSPNAYEIQ PDYSEYRRKQ QEKKD
References and
documentation are available.
Number of amino acids: 155
Molecular weight: 18186.77
Theoretical pI: 5.80
Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 26
Atomic composition:
Carbon C 752
Hydrogen H 1201
Nitrogen N 245
Oxygen O 266
Sulfur S 8
Formula: C
752H
1201N
245O
266S
8
Total number of atoms: 2472
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 11585
Abs 0.1% (=1 g/l) 0.637, assuming all pairs of Cys residues form cystines
Ext. coefficient 11460
Abs 0.1% (=1 g/l) 0.630, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 95.55
This classifies the protein as unstable.
Aliphatic index: 38.97
Grand average of hydropathicity (GRAVY): -1.620