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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSGSFWTSTQ RHHWQYTKAS LAKERQKLWL LECQLFPQGL NIVMDSKQNG IEQSITKNIP

70 80 90 100 110 120
ITHRDLHYDK DYNLRIYCYF LIMKLGRRLN IRQYALATAH IYLSRFLIKA SVREINLYML

130 140 150 160 170 180
VTTCVYLACK VEECPQYIRT LVSEARTLWP EFIPPDPTKV TEFEFYLLEE LESYLIVHHP

190 200 210 220 230 240
YQSLKQIVQV LKQPPFQITL SSDDLQNCWS LINDSYINDV HLLYPPHIIA VACLFITISI

250 260 270 280 290 300
HGKPTKGSSL ASAASEAIRD PKNSSSPVQI AFNRFMAESL VDLEEVMDTI QEQITLYDHW

310 320
DKYHEQWIKF LLHTLYLRPA SAI


References and documentation are available.

Number of amino acids: 323 Molecular weight: 37790.67 Theoretical pI: 6.67
Amino acid composition: 
Ala (A) 18 5.6% Arg (R) 14 4.3% Asn (N) 11 3.4% Asp (D) 14 4.3% Cys (C) 7 2.2% Gln (Q) 19 5.9% Glu (E) 19 5.9% Gly (G) 6 1.9% His (H) 13 4.0% Ile (I) 29 9.0% Leu (L) 39 12.1% Lys (K) 17 5.3% Met (M) 6 1.9% Phe (F) 12 3.7% Pro (P) 16 5.0% Ser (S) 25 7.7% Thr (T) 18 5.6% Trp (W) 7 2.2% Tyr (Y) 18 5.6% Val (V) 15 4.6% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 33 Total number of positively charged residues (Arg + Lys): 31 Atomic composition: Carbon C 1728 Hydrogen H 2669 Nitrogen N 445 Oxygen O 481 Sulfur S 13 Formula: C1728H2669N445O481S13 Total number of atoms: 5336 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 65695 Abs 0.1% (=1 g/l) 1.738, assuming all pairs of Cys residues form cystines Ext. coefficient 65320 Abs 0.1% (=1 g/l) 1.728, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 57.93 This classifies the protein as unstable. Aliphatic index: 101.15 Grand average of hydropathicity (GRAVY): -0.140