ProtParam
User-provided sequence:
10 20 30 40 50 60
MSGSFWTSTQ RHHWQYTKAS LAKERQKLWL LECQLFPQGL NIVMDSKQNG IEQSITKNIP
70 80 90 100 110 120
ITHRDLHYDK DYNLRIYCYF LIMKLGRRLN IRQYALATAH IYLSRFLIKA SVREINLYML
130 140 150 160 170 180
VTTCVYLACK VEECPQYIRT LVSEARTLWP EFIPPDPTKV TEFEFYLLEE LESYLIVHHP
190 200 210 220 230 240
YQSLKQIVQV LKQPPFQITL SSDDLQNCWS LINDSYINDV HLLYPPHIIA VACLFITISI
250 260 270 280 290 300
HGKPTKGSSL ASAASEAIRD PKNSSSPVQI AFNRFMAESL VDLEEVMDTI QEQITLYDHW
310 320
DKYHEQWIKF LLHTLYLRPA SAI
References and
documentation are available.
Number of amino acids: 323
Molecular weight: 37790.67
Theoretical pI: 6.67
Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 31
Atomic composition:
Carbon C 1728
Hydrogen H 2669
Nitrogen N 445
Oxygen O 481
Sulfur S 13
Formula: C
1728H
2669N
445O
481S
13
Total number of atoms: 5336
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 65695
Abs 0.1% (=1 g/l) 1.738, assuming all pairs of Cys residues form cystines
Ext. coefficient 65320
Abs 0.1% (=1 g/l) 1.728, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 57.93
This classifies the protein as unstable.
Aliphatic index: 101.15
Grand average of hydropathicity (GRAVY): -0.140