ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSGSHSNDED DVVQVPETSS PTKVASSSPL KPTSPTVPDA SVASLRSRFT FKPSDPSEGA 

        70         80         90        100        110        120 
HTSKPLPSGS PEVALVNLAR EFPDFSQTLV QAVFKSNSFN LQSARERLTR LRQQRQNWTW 

       130        140        150        160        170        180 
NKNASPKKSE TPPPVKKSLP LANTGRLSSI HGNINNKSSK ITVAKQKTSI FDRYSNVINQ 

       190        200        210        220        230        240 
KQYTFELPTN LNIDSEALSK LPVNYNKKRR LVRADQHPIG KSYESSATQL GSAREKLLAN 

       250        260        270        280        290        300 
RKYGRHANDN DEEEEESMMT DDDDASGDDY TESTPQINLD EQVLQFINDS DIVDLSDLSD 

       310        320        330        340        350        360 
TTMHKAQLIA SHRPYSSLNA FVNTNFNDKD TEENASNKRK RRAAASANES ERLLDKITQS 

       370        380        390        400        410        420 
IRGYNAIESV IKKCSSYGDL VTSQMKKWGV QVEGDNSELD LMNLGEDDDD DNDDGNNDNN 

       430        440        450        460        470        480 
NSNNNNTAGA DATSKEKEDT KAVVEGFDET SAEPTPAPAP APVERETKRI RNTTKPKVVE 

       490        500        510        520        530        540 
DEDDDVDLEA IDDELPQSEH EDDDYEEEDE DYNDEEEDVE YDDGDDDDDD DDEFVATRKN 

       550        560        570        580        590        600 
THVISTTSRN GRKPIVKFFK GKPRLLSPEI SLKDYQQTGI NWLNLLYQNK MSCILADDMG 

       610        620        630        640        650        660 
LGKTCQVISF FAYLKQINEP GPHLVVVPSS TLENWLREFQ KFAPALKIEP YYGSLQEREE 

       670        680        690        700        710        720 
LRDILERNAG KYDVIVTTYN LAAGNKYDVS FLKNRNFNVV VYDEGHMLKN STSERFAKLM 

       730        740        750        760        770        780 
KIRANFRLLL TGTPLQNNLK ELMSLLEFIM PNLFISKKES FDAIFKQRAK TTDDNKNHNP 

       790        800        810        820        830        840 
LLAQEAITRA KTMMKPFILR RRKDQVLKHL PPKHTHIQYC ELNAIQKKIY DKEIQIVLEH 

       850        860        870        880        890        900 
KRMIKDGELP KDAKEKSKLQ SSSSKNLIMA LRKASLHPLL FRNIYNDKII TKMSDAILDE 

       910        920        930        940        950        960 
PAYAENGNKE YIKEDMSYMT DFELHKLCCN FPNTLSKYQL HNDEWMQSGK IDALKKLLKT 

       970        980        990       1000       1010       1020 
IIVDKQEKVL IFSLFTQVLD ILEMVLSTLD YKFLRLDGST QVNDRQLLID KFYEDKDIPI 

      1030       1040       1050       1060       1070       1080 
FILSTKAGGF GINLVCANNV IIFDQSFNPH DDRQAADRAH RVGQTKEVNI TTLITKDSIE 

      1090       1100       1110       1120       1130 
EKIHQLAKNK LALDSYISED KKSQDVLESK VSDMLEDIIY DENSKPKGTK E 

References and documentation are available.

Number of amino acids: 1131

Molecular weight: 128506.79

Theoretical pI: 5.23

Amino acid composition: 
Ala (A)  66	  5.8%
Arg (R)  50	  4.4%
Asn (N)  82	  7.3%
Asp (D) 104	  9.2%
Cys (C)   7	  0.6%
Gln (Q)  46	  4.1%
Glu (E)  85	  7.5%
Gly (G)  38	  3.4%
His (H)  22	  1.9%
Ile (I)  64	  5.7%
Leu (L) 103	  9.1%
Lys (K) 102	  9.0%
Met (M)  22	  1.9%
Phe (F)  39	  3.4%
Pro (P)  50	  4.4%
Ser (S)  95	  8.4%
Thr (T)  63	  5.6%
Trp (W)   6	  0.5%
Tyr (Y)  32	  2.8%
Val (V)  55	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 189
Total number of positively charged residues (Arg + Lys): 152

Atomic composition:

Carbon      C	      5617
Hydrogen    H	      8929
Nitrogen    N	      1561
Oxygen      O	      1828
Sulfur      S	        29

Formula: C5617H8929N1561O1828S29
Total number of atoms: 17964

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    81055
Abs 0.1% (=1 g/l)   0.631, assuming all pairs of Cys residues form cystines


Ext. coefficient    80680
Abs 0.1% (=1 g/l)   0.628, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.66
This classifies the protein as unstable.



Aliphatic index: 77.52

Grand average of hydropathicity (GRAVY): -0.767