ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSGSLTLLKE KHIRYIESLD TKKHNFEYWL TEHLRLNGIY WGLTALCVLD SPETFVKEEV 

        70         80         90        100        110        120 
ISFVLSCWDD KYGAFAPFPR HDAHLLTTLS AVQILATYDA LDVLGKDRKV RLISFIRGNQ 

       130        140        150        160        170        180 
LEDGSFQGDR FGEVDTRFVY TALSALSILG ELTSEVVDPA VDFVLKCYNF DGGFGLCPNA 

       190        200        210        220        230        240 
ESHAAQAFTC LGALAIANKL DMLSDDQLEE IGWWLCERQL PEGGLNGRPS KLPDVCYSWW 

       250        260        270        280        290        300 
VLSSLAIIGR LDWINYEKLT EFILKCQDEK KGGISDRPEN EVDVFHTVFG VAGLSLMGYD 

       310        320 
NLVPIDPIYC MPKSVTSKFK KYPYK 

References and documentation are available.

Number of amino acids: 325

Molecular weight: 36666.00

Theoretical pI: 5.03

Amino acid composition: 
Ala (A)  19	  5.8%
Arg (R)  12	  3.7%
Asn (N)  10	  3.1%
Asp (D)  24	  7.4%
Cys (C)   9	  2.8%
Gln (Q)   7	  2.2%
Glu (E)  21	  6.5%
Gly (G)  24	  7.4%
His (H)   7	  2.2%
Ile (I)  17	  5.2%
Leu (L)  42	 12.9%
Lys (K)  20	  6.2%
Met (M)   4	  1.2%
Phe (F)  17	  5.2%
Pro (P)  13	  4.0%
Ser (S)  22	  6.8%
Thr (T)  15	  4.6%
Trp (W)   8	  2.5%
Tyr (Y)  13	  4.0%
Val (V)  21	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 32

Atomic composition:

Carbon      C	      1670
Hydrogen    H	      2560
Nitrogen    N	       420
Oxygen      O	       483
Sulfur      S	        13

Formula: C1670H2560N420O483S13
Total number of atoms: 5146

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    63870
Abs 0.1% (=1 g/l)   1.742, assuming all pairs of Cys residues form cystines


Ext. coefficient    63370
Abs 0.1% (=1 g/l)   1.728, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 30.41
This classifies the protein as stable.



Aliphatic index: 95.38

Grand average of hydropathicity (GRAVY): -0.055