ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSGSTESKKQ PRRRFIGRKS GNSNNDKLTT VAENGNEIIH KQKSRIALGR SVNHVPEDIL 

        70         80         90        100        110        120 
NDKELNEAIK LLPSNYNFEI HKTVWNIRKY NAKRIALQMP EGLLIYSLII SDILEQFCGV 

       130        140        150        160        170        180 
ETLVMGDVSY GACCIDDFTA RALDCDFIVH YAHSCLVPID VTKIKVLYVF VTINIQEDHI 

       190        200        210        220        230        240 
IKTLQKNFPK GSRIATFGTI QFNPAVHSVR DKLLNDEEHM LYIIPPQIKP LSRGEVLGCT 

       250        260        270        280        290        300 
SERLDKEQYD AMVFIGDGRF HLESAMIHNP EIPAFKYDPY NRKFTREGYD QKQLVEVRAE 

       310        320        330        340        350        360 
AIEVARKGKV FGLILGALGR QGNLNTVKNL EKNLIAAGKT VVKIILSEVF PQKLAMFDQI 

       370        380        390        400        410        420 
DVFVQVACPR LSIDWGYAFN KPLLTPYEAS VLLKKDVMFS EKYYPMDYYE AKGYGRGETP 


KHAIE 

References and documentation are available.

Number of amino acids: 425

Molecular weight: 48310.81

Theoretical pI: 8.46

Amino acid composition: 
Ala (A)  26	  6.1%
Arg (R)  21	  4.9%
Asn (N)  23	  5.4%
Asp (D)  23	  5.4%
Cys (C)   7	  1.6%
Gln (Q)  15	  3.5%
Glu (E)  29	  6.8%
Gly (G)  26	  6.1%
His (H)  11	  2.6%
Ile (I)  37	  8.7%
Leu (L)  37	  8.7%
Lys (K)  35	  8.2%
Met (M)   9	  2.1%
Phe (F)  19	  4.5%
Pro (P)  19	  4.5%
Ser (S)  21	  4.9%
Thr (T)  17	  4.0%
Trp (W)   2	  0.5%
Tyr (Y)  18	  4.2%
Val (V)  30	  7.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 56

Atomic composition:

Carbon      C	      2177
Hydrogen    H	      3445
Nitrogen    N	       585
Oxygen      O	       624
Sulfur      S	        16

Formula: C2177H3445N585O624S16
Total number of atoms: 6847

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    38195
Abs 0.1% (=1 g/l)   0.791, assuming all pairs of Cys residues form cystines


Ext. coefficient    37820
Abs 0.1% (=1 g/l)   0.783, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.05
This classifies the protein as unstable.



Aliphatic index: 94.49

Grand average of hydropathicity (GRAVY): -0.255