ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSGWNVNASS FVPNANARPF VPGQPYTPQP EQAAPEPTED WEAQADTSPA AVQPEVAEPV 

        70         80         90        100        110        120 
AVQESAPVAP VSATEAPKKE PTPEEDLVAP LAKKFQRTVY VDDGTHKEHI NMVFVGHVDA 

       130        140        150        160        170        180 
GKSTIGGQLM FLTGMVDKRT LEKYEREAKE KGRESWYLSW CMDTNDEERE KGKTVEVGRA 

       190        200        210        220        230        240 
YFETEKRHFT ILDAPGHKSF VPNMIVGANQ ADLAVLVISA RRGEFETGFD RGGQTREHSM 

       250        260        270        280        290        300 
LVKTAGVKHL VILVNKMDDP TVKWEEERFK EIEGKLTPFL RKLGFNPKTD ITYVPCSGLT 

       310        320        330        340        350        360 
GAFIKDRPTG SEGNWYSGPC FIEFIDVLLP SYKRDFNGPV RCTVAEKYSE MGTVIIGKME 

       370        380        390        400        410        420 
SGCVQKGDTL VVMPNKQPVQ VLQIWADDVE TERVVAGDNI KFKLKGIEEN ELQGGFIICS 

       430        440        450        460        470        480 
PDSLAKTGRV FDAEVLVLEH RSIIASGYSC VLHIQSAVEE VTVKGVIATI DKKTGEKKRA 

       490        500        510        520        530 
KFVKQDEKCI MRLESPEPFV LEPFKEYPYL GRFTLRDEGK TIAIGKVLKV VE 

References and documentation are available.

Number of amino acids: 532

Molecular weight: 59162.55

Theoretical pI: 5.50

Amino acid composition: 
Ala (A)  37	  7.0%
Arg (R)  24	  4.5%
Asn (N)  15	  2.8%
Asp (D)  27	  5.1%
Cys (C)   8	  1.5%
Gln (Q)  17	  3.2%
Glu (E)  53	 10.0%
Gly (G)  41	  7.7%
His (H)   9	  1.7%
Ile (I)  26	  4.9%
Leu (L)  31	  5.8%
Lys (K)  44	  8.3%
Met (M)  12	  2.3%
Phe (F)  24	  4.5%
Pro (P)  34	  6.4%
Ser (S)  25	  4.7%
Thr (T)  33	  6.2%
Trp (W)   7	  1.3%
Tyr (Y)  12	  2.3%
Val (V)  53	 10.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 80
Total number of positively charged residues (Arg + Lys): 68

Atomic composition:

Carbon      C	      2642
Hydrogen    H	      4161
Nitrogen    N	       705
Oxygen      O	       795
Sulfur      S	        20

Formula: C2642H4161N705O795S20
Total number of atoms: 8323

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    56880
Abs 0.1% (=1 g/l)   0.961, assuming all pairs of Cys residues form cystines


Ext. coefficient    56380
Abs 0.1% (=1 g/l)   0.953, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.01
This classifies the protein as unstable.



Aliphatic index: 77.63

Grand average of hydropathicity (GRAVY): -0.380