ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSHPIQFVNA NNSDKSHQLG GQYSIPQDLR ENLQKEAARI GENEKDVLQE KMETRTVQNR 

        70         80         90        100        110        120 
EDSYHKRRFD MKFEPDSDTQ TVTSSENTQD AVVPRKRKSR WDVKGYEPPD ESSTAVKENS 

       130        140        150        160        170        180 
DSALVNVEGI HDLMFFKPSD HKYFADVISK KPIDELNKDE KKERTLSMLL LKIKNGNTAS 

       190        200        210        220        230        240 
RRTSMRILTD KAVTFGPEMI FNRLLPILLD RSLEDQERHL MIKTIDRVLY QLGDLTKPYV 

       250        260        270        280        290        300 
HKILVVAAPL LIDEDPMVRS TGQEIITNLS TVAGLKTILT VMRPDIENED EYVRNVTSRA 

       310        320        330        340        350        360 
AAVVAKALGV NQLLPFINAA CHSRKSWKAR HTGIKIVQQI GILLGIGVLN HLTGLMSCIK 

       370        380        390        400        410        420 
DCLMDDHVPV RIVTAHTLST LAENSYPYGI EVFNVVLEPL WKGIRSHRGK VLSSFLKAVG 

       430        440        450        460        470        480 
SMIPLMDPEY AGYYTTEAMR IIRREFDSPD DEMKKTILLV LQKCSAVESI TPKFLREEIA 

       490        500        510        520        530        540 
PEFFQKFWVR RVALDRPLNK VVTYTTVTLA KKLGCSYTID KLLTPLRDEA EPFRTMAVHA 

       550        560        570        580        590        600 
VTRTVNLLGT ADLDERLETR LIDALLIAFQ EQTNSDSIIF KGFGAVTVSL DIRMKPFLAP 

       610        620        630        640        650        660 
IVSTILNHLK HKTPLVRQHA ADLCAILIPV IKNCHEFEML NKLNIILYES LGEVYPEVLG 

       670        680        690        700        710        720 
SIINAMYCIT SVMDLDKLQP PINQILPTLT PILRNKHRKV EVNTIKFVGL IGKLAPTYAP 

       730        740        750        760        770        780 
PKEWMRICFE LLELLKSTNK EIRRSANATF GFIAEAIGPH DVLVALLNNL KVQERQLRVC 

       790        800        810        820        830        840 
TAVAIGIVAK VCGPYNVLPV IMNEYTTPET NVQNGVLKAM SFMFEYIGNM SKDYIYFITP 

       850        860        870        880        890        900 
LLEDALTDRD LVHRQTASNV ITHLALNCSG TGHEDAFIHL MNLLIPNIFE TSPHAIMRIL 

       910        920        930        940        950        960 
EGLEALSQAL GPGLFMNYIW AGLFHPAKNV RKAFWRVYNN MYVMYQDAMV PFYPVTPDNN 

       970 
EEYIEELDLV L 

References and documentation are available.

Number of amino acids: 971

Molecular weight: 110027.79

Theoretical pI: 6.83

Amino acid composition: 
Ala (A)  63	  6.5%
Arg (R)  52	  5.4%
Asn (N)  51	  5.3%
Asp (D)  52	  5.4%
Cys (C)  12	  1.2%
Gln (Q)  28	  2.9%
Glu (E)  64	  6.6%
Gly (G)  41	  4.2%
His (H)  26	  2.7%
Ile (I)  73	  7.5%
Leu (L) 109	 11.2%
Lys (K)  61	  6.3%
Met (M)  32	  3.3%
Phe (F)  35	  3.6%
Pro (P)  50	  5.1%
Ser (S)  50	  5.1%
Thr (T)  62	  6.4%
Trp (W)   7	  0.7%
Tyr (Y)  29	  3.0%
Val (V)  74	  7.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 116
Total number of positively charged residues (Arg + Lys): 113

Atomic composition:

Carbon      C	      4936
Hydrogen    H	      7912
Nitrogen    N	      1326
Oxygen      O	      1424
Sulfur      S	        44

Formula: C4936H7912N1326O1424S44
Total number of atoms: 15642

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    82460
Abs 0.1% (=1 g/l)   0.749, assuming all pairs of Cys residues form cystines


Ext. coefficient    81710
Abs 0.1% (=1 g/l)   0.743, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.15
This classifies the protein as stable.



Aliphatic index: 101.69

Grand average of hydropathicity (GRAVY): -0.109