ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSHSGAAIFE KVSGIIAINE DVSPAELTWR STDGDKVHTV VLSTIDKLQA TPASSEKMML 

        70         80         90        100        110        120 
RLIGKVDESK KRKDNEGNEV VPKPQRHMFS FNNRTVMDNI KMTLQQIISR YKDADIYEEK 

       130        140        150        160        170        180 
RRREESAQHT ETPMSSSSVT AGTPTPHLDT PQLNNGAPLI NTAKLDDSLS KEKLLTNLKL 

       190        200        210        220        230        240 
QQSLLKGNKV LMKVFQETVI NAGLPPSEFW STRIPLLRAF ALSTSQKVGP YNVLSTIKPV 

       250        260        270        280        290        300 
ASSENKVNVN LSREKILNIF ENYPIVKKAY TDNVPKNFKE PEFWARFFSS KLFRKLRGEK 

       310        320        330        340        350        360 
IMQNDRGDVI IDRYLTLDQE FDRKDDDMLL HPVKKIIDLD GNIQDDPVVR GNRPDFTMQP 

       370        380        390        400        410        420 
GVDINGNSDG TVDILKGMNR LSEKMIMALK NEYSRTNLQN KSNITNDEED EDNDERNELK 

       430        440        450        460        470        480 
IDDLNESYKT NYAIIHLKRN AHEKTTDNDA KSSADSIKNA DLKVSNQQML QQLSLVMDNL 

       490        500        510        520        530        540 
INKLDLNQVV PNNEVSNKIN KRVITAIKIN AKQAKHNNVN SALGSFVDNT SQANELEVKS 

       550        560        570        580        590        600 
TLPIDLLESC RMLHTTCCEF LKHFYIHFQS GEQKQASTVK KLYNHLKDCI EKLNELFQDV 

       610        620        630        640 
LNGDGESMSN TCTAYLKPVL NSITLATHKY DEYFNEYNNN SN 

References and documentation are available.

Number of amino acids: 642

Molecular weight: 72894.46

Theoretical pI: 6.44

Amino acid composition: 
Ala (A)  31	  4.8%
Arg (R)  26	  4.0%
Asn (N)  62	  9.7%
Asp (D)  47	  7.3%
Cys (C)   5	  0.8%
Gln (Q)  26	  4.0%
Glu (E)  42	  6.5%
Gly (G)  22	  3.4%
His (H)  14	  2.2%
Ile (I)  40	  6.2%
Leu (L)  61	  9.5%
Lys (K)  59	  9.2%
Met (M)  18	  2.8%
Phe (F)  20	  3.1%
Pro (P)  24	  3.7%
Ser (S)  51	  7.9%
Thr (T)  38	  5.9%
Trp (W)   3	  0.5%
Tyr (Y)  15	  2.3%
Val (V)  38	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 89
Total number of positively charged residues (Arg + Lys): 85

Atomic composition:

Carbon      C	      3181
Hydrogen    H	      5124
Nitrogen    N	       898
Oxygen      O	      1013
Sulfur      S	        23

Formula: C3181H5124N898O1013S23
Total number of atoms: 10239

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    39100
Abs 0.1% (=1 g/l)   0.536, assuming all pairs of Cys residues form cystines


Ext. coefficient    38850
Abs 0.1% (=1 g/l)   0.533, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.61
This classifies the protein as stable.



Aliphatic index: 83.35

Grand average of hydropathicity (GRAVY): -0.652