ProtParam
User-provided sequence:
10 20 30 40 50 60
MSHSLTSVFQ KIDSLKPQFF SRLTKAIQIP AVSSDESLRS KVFDKAKFIS EQLSQSGFHD
70 80 90 100 110 120
IKMVDLGIQP PPISTPNLSL PPVILSRFGS DPSKKTVLVY GHYDVQPAQL EDGWDTEPFK
130 140 150 160 170 180
LVIDEAKGIM KGRGVTDDTG PLLSWINVVD AFKASGQEFP VNLVTCFEGM EESGSLKLDE
190 200 210 220 230 240
LIKKEANGYF KGVDAVCISD NYWLGTKKPV LTYGLRGCNY YQTIIEGPSA DLHSGIFGGV
250 260 270 280 290 300
VAEPMIDLMQ VLGSLVDSKG KILIDGIDEM VAPLTEKEKA LYKDIEFSVE ELNAATGSKT
310 320 330 340 350 360
SLYDKKEDIL MHRWRYPSLS IHGVEGAFSA QGAKTVIPAK VFGKFSIRTV PDMDSEKLTS
370 380 390 400 410 420
LVQKHCDAKF KSLNSPNKCR TELIHDGAYW VSDPFNAQFT AAKKATKLVY GVDPDFTREG
430 440 450 460 470 480
GSIPITLTFQ DALNTSVLLL PMGRGDDGAH SINEKLDISN FVGGMKTMAA YLQYYSESPE
N
References and
documentation are available.
Number of amino acids: 481
Molecular weight: 52871.38
Theoretical pI: 5.43
Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 51
Atomic composition:
Carbon C 2377
Hydrogen H 3725
Nitrogen N 607
Oxygen O 720
Sulfur S 17
Formula: C
2377H
3725N
607O
720S
17
Total number of atoms: 7446
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 50100
Abs 0.1% (=1 g/l) 0.948, assuming all pairs of Cys residues form cystines
Ext. coefficient 49850
Abs 0.1% (=1 g/l) 0.943, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.62
This classifies the protein as stable.
Aliphatic index: 85.93
Grand average of hydropathicity (GRAVY): -0.202