ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSHSRLFARL LLQQAGVRHC YSGSRNNLYI NKNTKVICQG FTGKQGTFHS QQSLDYGSQL 

        70         80         90        100        110        120 
VGGVSPGKGG KTHLGLPVFN SVKEAKDGTG AEATVIYVPP PFAAAAIIEA IDAEMPLAVC 

       130        140        150        160        170        180 
ITEGIPQQDM VRVKHRLLRQ NKTRLVGPNC PGVINPGECK IGIMPGHIHK KGRIGIVSRS 

       190        200        210        220        230        240 
GTLPYEAVHQ TTQVGLGQSL CIGIGGDPFN GTNFIDCLEV FLQDPKTEGI ILIGEIGGDA 

       250        260        270        280        290        300 
EENAAEYLKQ HNSGANAKPV VSFIAGLTAP PGRRMGHAGA IIAGGKGGAK EKIAALQSAG 

       310        320 
VVVSMSPAQL GSTIFKEFEK RKML 

References and documentation are available.

Number of amino acids: 324

Molecular weight: 34169.43

Theoretical pI: 9.29

Amino acid composition: 
Ala (A)  28	  8.6%
Arg (R)  13	  4.0%
Asn (N)  13	  4.0%
Asp (D)   8	  2.5%
Cys (C)   7	  2.2%
Gln (Q)  17	  5.2%
Glu (E)  16	  4.9%
Gly (G)  44	 13.6%
His (H)  10	  3.1%
Ile (I)  26	  8.0%
Leu (L)  24	  7.4%
Lys (K)  21	  6.5%
Met (M)   7	  2.2%
Phe (F)  11	  3.4%
Pro (P)  18	  5.6%
Ser (S)  18	  5.6%
Thr (T)  15	  4.6%
Trp (W)   0	  0.0%
Tyr (Y)   6	  1.9%
Val (V)  22	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 24
Total number of positively charged residues (Arg + Lys): 34

Atomic composition:

Carbon      C	      1508
Hydrogen    H	      2438
Nitrogen    N	       434
Oxygen      O	       442
Sulfur      S	        14

Formula: C1508H2438N434O442S14
Total number of atoms: 4836

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     9315
Abs 0.1% (=1 g/l)   0.273, assuming all pairs of Cys residues form cystines


Ext. coefficient     8940
Abs 0.1% (=1 g/l)   0.262, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.93
This classifies the protein as unstable.



Aliphatic index: 88.52

Grand average of hydropathicity (GRAVY): -0.086