ProtParam
User-provided sequence:
10 20 30 40 50 60
MSHSYASSSS SSSYRRMFGG PGYMTPPMSR GVFGRASTGG SGSSRVSSRV YEVSKSSTSS
70 80 90 100 110 120
PGFSGYRASS YSMPNLSAGY TRSYGGMGET LDFSLADALN QEFLHTRTNE KAELQHLNDR
130 140 150 160 170 180
FANYIEKVRM LEQQNQVLGV EVERLRGREP TRIADLYEDE MRELRREVEV VTNHRSRVEV
190 200 210 220 230 240
ERDNLADDLQ KLKLRLQEEI ALREEAENNL SAFRADVDAA TLARLDLERR IETLQEEIAF
250 260 270 280 290 300
LKKIHEEEIR ELQAQMQETQ VQIQMDMSKP DLTAALRDIR AQYEGIAAKN IAEAEDWYKS
310 320 330 340 350 360
KVSDLNQAVS KNNEALKQSK LETMEYRHQI QSYTCEIDSL KGSNESLMRQ MRDMEDRHGR
370 380 390 400 410 420
EAGALQDTIA RLEAEIANMK DEMARHLREY QDLLNVKMAL DVEIATYRKL LEGEESRIVM
430 440 450 460 470
PMQSYSTISF RETSPEHQQR ASEMHSKKTV LIKTIETRDG EVVSESTQHQ QDLM
References and
documentation are available.
Number of amino acids: 474
Molecular weight: 54274.66
Theoretical pI: 5.36
Total number of negatively charged residues (Asp + Glu): 80
Total number of positively charged residues (Arg + Lys): 64
Atomic composition:
Carbon C 2314
Hydrogen H 3734
Nitrogen N 692
Oxygen O 768
Sulfur S 23
Formula: C
2314H
3734N
692O
768S
23
Total number of atoms: 7531
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 30830
Abs 0.1% (=1 g/l) 0.568, assuming all pairs of Cys residues form cystines
Ext. coefficient 30830
Abs 0.1% (=1 g/l) 0.568, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 51.36
This classifies the protein as unstable.
Aliphatic index: 72.89
Grand average of hydropathicity (GRAVY): -0.773