ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSHVAVENAL GLDQQFAGLD LNSSDNQSGG STASKGRYIP PHLRNREATK GFYDKDSSGW 

        70         80         90        100        110        120 
SSSKDKDAYS SFGSRGDSRG KSSFFGDRGS GSRGRFDDRG RGDYDGIGGR GDRSGFGKFE 

       130        140        150        160        170        180 
RGGNSRWCDK SDEDDWSKPL PPSERLEQEL FSGGNTGINF EKYDDIPVEA TGNNCPPHIE 

       190        200        210        220        230        240 
SFSDVEMGEI IMGNIELTRY TRPTPVQKHA IPIIKEKRDL MACAQTGSGK TAAFLLPILS 

       250        260        270        280        290        300 
QIYADGPGEA LRAMKENGRY GRRKQYPISL VLAPTRELAV QIYEEARKFS YRSRVRPCVV 

       310        320        330        340        350        360 
YGGAEIGQQI RDLERGCHLL VATPGRLVDM MERGKIGLDF CKYLVLDEAD RMLDMGFEPQ 

       370        380        390        400        410        420 
IRRIVEQDTM PPKGVRHTMM FSATFPKEIQ MLARDFLDEY IFLAVGRVGS TSENITQKVV 

       430        440        450        460        470        480 
WVEEIDKRSF LLDLLNATGK DSLTLVFVET KKGADSLEDF LYHEGYACTS IHGDRSQRDR 

       490        500        510        520        530        540 
EEALHQFRSG KSPILVATAV AARGLDISNV KHVINFDLPS DIEEYVHRIG RTGRVGNLGL 

       550        560        570        580        590        600 
ATSFFNERNI NITKDLLDLL VEAKQEVPSW LENMAFEHHY KGSSRGRSKS SRFSGGFGAR 

       610        620        630        640        650        660 
DYRQSSGASS SSFSSSRASS SRSGGGGHGG SRGFGGGGYG GFYNSDGYGG NYNSQGVDWW 


GN 

References and documentation are available.

Number of amino acids: 662

Molecular weight: 73101.40

Theoretical pI: 6.73

Amino acid composition: 
Ala (A)  38	  5.7%
Arg (R)  55	  8.3%
Asn (N)  24	  3.6%
Asp (D)  47	  7.1%
Cys (C)   7	  1.1%
Gln (Q)  20	  3.0%
Glu (E)  42	  6.3%
Gly (G)  81	 12.2%
His (H)  14	  2.1%
Ile (I)  32	  4.8%
Leu (L)  47	  7.1%
Lys (K)  32	  4.8%
Met (M)  14	  2.1%
Phe (F)  32	  4.8%
Pro (P)  24	  3.6%
Ser (S)  68	 10.3%
Thr (T)  24	  3.6%
Trp (W)   7	  1.1%
Tyr (Y)  23	  3.5%
Val (V)  31	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 89
Total number of positively charged residues (Arg + Lys): 87

Atomic composition:

Carbon      C	      3188
Hydrogen    H	      4960
Nitrogen    N	       938
Oxygen      O	      1000
Sulfur      S	        21

Formula: C3188H4960N938O1000S21
Total number of atoms: 10107

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    73145
Abs 0.1% (=1 g/l)   1.001, assuming all pairs of Cys residues form cystines


Ext. coefficient    72770
Abs 0.1% (=1 g/l)   0.995, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.35
This classifies the protein as unstable.



Aliphatic index: 65.86

Grand average of hydropathicity (GRAVY): -0.613