ProtParam
User-provided sequence:
10 20 30 40 50 60
MSIAEFAYKE KPETLVLFDV DGTLTPARLT VSEEVRKTLA KLRNKCCIGF VGGSDLSKQL
70 80 90 100 110 120
EQLGPNVLDE FDYSFSENGL TAYRLGKELA SQSFINWLGE EKYNKLAVFI LRYLSEIDLP
130 140 150 160 170 180
KRRGTFLEFR NGMINVSPIG RNASTEERNE FERYDKEHQI RAKFVEALKK EFPDYGLTFS
190 200 210 220 230 240
IGGQISFDVF PAGWDKTYCL QHVEKDGFKE IHFFGDKTMV GGNDYEIFVD ERTIGHSVQS
250
PDDTVKILTE LFNL
References and
documentation are available.
Number of amino acids: 254
Molecular weight: 29062.91
Theoretical pI: 5.14
Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 32
Atomic composition:
Carbon C 1314
Hydrogen H 2022
Nitrogen N 340
Oxygen O 393
Sulfur S 6
Formula: C
1314H
2022N
340O
393S
6
Total number of atoms: 4075
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 24535
Abs 0.1% (=1 g/l) 0.844, assuming all pairs of Cys residues form cystines
Ext. coefficient 24410
Abs 0.1% (=1 g/l) 0.840, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.61
This classifies the protein as unstable.
Aliphatic index: 81.34
Grand average of hydropathicity (GRAVY): -0.386