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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSIAEFAYKE KPETLVLFDV DGTLTPARLT VSEEVRKTLA KLRNKCCIGF VGGSDLSKQL

70 80 90 100 110 120
EQLGPNVLDE FDYSFSENGL TAYRLGKELA SQSFINWLGE EKYNKLAVFI LRYLSEIDLP

130 140 150 160 170 180
KRRGTFLEFR NGMINVSPIG RNASTEERNE FERYDKEHQI RAKFVEALKK EFPDYGLTFS

190 200 210 220 230 240
IGGQISFDVF PAGWDKTYCL QHVEKDGFKE IHFFGDKTMV GGNDYEIFVD ERTIGHSVQS

250
PDDTVKILTE LFNL


References and documentation are available.

Number of amino acids: 254 Molecular weight: 29062.91 Theoretical pI: 5.14
Amino acid composition: 
Ala (A) 11 4.3% Arg (R) 13 5.1% Asn (N) 11 4.3% Asp (D) 16 6.3% Cys (C) 3 1.2% Gln (Q) 7 2.8% Glu (E) 25 9.8% Gly (G) 20 7.9% His (H) 4 1.6% Ile (I) 14 5.5% Leu (L) 25 9.8% Lys (K) 19 7.5% Met (M) 3 1.2% Phe (F) 20 7.9% Pro (P) 8 3.1% Ser (S) 15 5.9% Thr (T) 14 5.5% Trp (W) 2 0.8% Tyr (Y) 9 3.5% Val (V) 15 5.9% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 41 Total number of positively charged residues (Arg + Lys): 32 Atomic composition: Carbon C 1314 Hydrogen H 2022 Nitrogen N 340 Oxygen O 393 Sulfur S 6 Formula: C1314H2022N340O393S6 Total number of atoms: 4075 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 24535 Abs 0.1% (=1 g/l) 0.844, assuming all pairs of Cys residues form cystines Ext. coefficient 24410 Abs 0.1% (=1 g/l) 0.840, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 41.61 This classifies the protein as unstable. Aliphatic index: 81.34 Grand average of hydropathicity (GRAVY): -0.386