ProtParam
User-provided sequence:
10 20 30 40 50 60
MSICEQYYPE EPTKPTVKTE SIPGPESQKQ LKELGEVFDT RPAYFLADYE KSLGNYITDV
70 80 90 100 110 120
DGNTYLDLYA QISSIALGYN NPALIKAAQS PEMIRALVDR PALGNFPSKD LDKILKQILK
130 140 150 160 170 180
SAPKGQDHVW SGLSGADANE LAFKAAFIYY RAKQRGYDAD FSEKENLSVM DNDAPGAPHL
190 200 210 220 230 240
AVLSFKRAFH GRLFASGSTT CSKPIHKLDF PAFHWPHAEY PSYQYPLDEN SDANRKEDDH
250 260 270 280 290 300
CLAIVEELIK TWSIPVAALI IEPIQSEGGD NHASKYFLQK LRDITLKYNV VYIIDEVQTG
310 320 330 340 350 360
VGATGKLWCH EYADIQPPVD LVTFSKKFQS AGYFFHDPKF IPNKPYRQFN TWCGEPARMI
370 380 390 400 410 420
IAGAIGQEIS DKKLTEQCSR VGDYLFKKLE GLQKKYPENF QNLRGKGRGT FIAWDLPTGE
430 440 450 460 470
KRDLLLKKLK LNGCNVGGCA VHAVRLRPSL TFEEKHADIF IEALAKSVNE L
References and
documentation are available.
Number of amino acids: 471
Molecular weight: 52946.24
Theoretical pI: 6.34
Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 57
Atomic composition:
Carbon C 2399
Hydrogen H 3694
Nitrogen N 630
Oxygen O 700
Sulfur S 12
Formula: C
2399H
3694N
630O
700S
12
Total number of atoms: 7435
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 66280
Abs 0.1% (=1 g/l) 1.252, assuming all pairs of Cys residues form cystines
Ext. coefficient 65780
Abs 0.1% (=1 g/l) 1.242, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.53
This classifies the protein as stable.
Aliphatic index: 81.46
Grand average of hydropathicity (GRAVY): -0.420