ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSIDNKLFVT EEDEEDRTQD RADVEDESND IDMIADENGT NSAIANEQEE KSEEVKAEDD 

        70         80         90        100        110        120 
TGEEEEDDPV IEEFPLKISG EEESLHVFQY ANRPRLVGRK PAEHPFISAA RYKPKSHLWE 

       130        140        150        160        170        180 
IDIPLDEQAF YNKDKAESEW NGVNVQTLKG VGVENNGQYA AFVKDMQVYL VPIERVAQLK 

       190        200        210        220        230        240 
PFFKYIDDAN VTRKQEDARR NPNPSSQRAQ VVTMSVKSVN DPSQNRLTGS LLAHKVADEE 

       250        260        270        280 
ANIELTWAEG TFEQFKDTIV KEAEDKTLVA LEKQEDYIDN LV 

References and documentation are available.

Number of amino acids: 282

Molecular weight: 32142.22

Theoretical pI: 4.39

Amino acid composition: 
Ala (A)  23	  8.2%
Arg (R)  12	  4.3%
Asn (N)  18	  6.4%
Asp (D)  26	  9.2%
Cys (C)   0	  0.0%
Gln (Q)  14	  5.0%
Glu (E)  39	 13.8%
Gly (G)  10	  3.5%
His (H)   4	  1.4%
Ile (I)  14	  5.0%
Leu (L)  16	  5.7%
Lys (K)  20	  7.1%
Met (M)   4	  1.4%
Phe (F)  10	  3.5%
Pro (P)  12	  4.3%
Ser (S)  15	  5.3%
Thr (T)  12	  4.3%
Trp (W)   3	  1.1%
Tyr (Y)   7	  2.5%
Val (V)  23	  8.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 32

Atomic composition:

Carbon      C	      1400
Hydrogen    H	      2181
Nitrogen    N	       381
Oxygen      O	       479
Sulfur      S	         4

Formula: C1400H2181N381O479S4
Total number of atoms: 4445

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    26930
Abs 0.1% (=1 g/l)   0.838

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.05
This classifies the protein as unstable.



Aliphatic index: 73.30

Grand average of hydropathicity (GRAVY): -0.859