ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSILLRPSSS PSLCSSLKLF RLSSPDSLID AAVLRNRTKP SQSFRMEVVS SNSTCLSSIS 

        70         80         90        100        110        120 
VGEDFPSEYE QWLPVPDPES RRRAGVLLHP TSFRGPHGIG DLGEEAFRFI DWLHSTGCSV 

       130        140        150        160        170        180 
WQVLPLVPPD EGGSPYAGQD ANCGNTLLIS LDELVKDGLL IKDELPQPID ADSVNYQTAN 

       190        200        210        220        230        240 
KLKSPLITKA AKRLIDGNGE LKSKLLDFRN DPSISCWLED AAYFAAIDNT LNAYSWFEWP 

       250        260        270        280        290        300 
EPLKNRHLSA LEAIYESQKE FIDLFIAKQF LFQRQWQKVR EYARRQGVDI MGDMPIYVGY 

       310        320        330        340        350        360 
HSADVWANKK HFLLNKKGFP LLVSGVPPDL FSETGQLWGS PLYDWKAMES DQYSWWVNRI 

       370        380        390        400        410        420 
RRAQDLYDEC RIDHFRGFAG FWAVPSEAKV AMVGRWKVGP GKSLFDAISK GVGKIKIIAE 

       430        440        450        460        470        480 
DLGVITKDVV ELRKSIGAPG MAVLQFAFGG GADNPHLPHN HEVNQVVYSG THDNDTIRGW 

       490        500        510        520        530        540 
WDTLDQEEKS KAMKYLSIAG EDDISWSVIQ AAFSSTAQTA IIPMQDILGL GSSARMNTPA 

       550        560        570 
TEVGNWGWRI PSSTSFDNLE TESDRLRDLL SLYGRL 

References and documentation are available.

Number of amino acids: 576

Molecular weight: 64411.95

Theoretical pI: 5.44

Amino acid composition: 
Ala (A)  41	  7.1%
Arg (R)  30	  5.2%
Asn (N)  21	  3.6%
Asp (D)  42	  7.3%
Cys (C)   6	  1.0%
Gln (Q)  21	  3.6%
Glu (E)  31	  5.4%
Gly (G)  42	  7.3%
His (H)  11	  1.9%
Ile (I)  33	  5.7%
Leu (L)  59	 10.2%
Lys (K)  30	  5.2%
Met (M)  10	  1.7%
Phe (F)  24	  4.2%
Pro (P)  33	  5.7%
Ser (S)  57	  9.9%
Thr (T)  19	  3.3%
Trp (W)  19	  3.3%
Tyr (Y)  15	  2.6%
Val (V)  32	  5.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 73
Total number of positively charged residues (Arg + Lys): 60

Atomic composition:

Carbon      C	      2897
Hydrogen    H	      4461
Nitrogen    N	       779
Oxygen      O	       856
Sulfur      S	        16

Formula: C2897H4461N779O856S16
Total number of atoms: 9009

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   127225
Abs 0.1% (=1 g/l)   1.975, assuming all pairs of Cys residues form cystines


Ext. coefficient   126850
Abs 0.1% (=1 g/l)   1.969, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.66
This classifies the protein as unstable.



Aliphatic index: 85.52

Grand average of hydropathicity (GRAVY): -0.300