ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSILSKLSQI SIKSAVQSSK HCFSSYPWLH KPISRTTILK TWRICGFGAG LGAVTLRKAA 

        70         80         90        100        110        120 
CAPKLSKRID HLRTTEDQNA SMTAGELWNL IKPFFGWFFA AVVCAILSAY INIQIPLCLG 

       130        140        150        160        170        180 
DLVNGIVKII KDESNNLRSH FEQLKPSALH LMTLYVAQSA LTFLYITFLT VLGERMATKM 

       190        200        210        220        230        240 
RSDLFQKLLH HDMAFFDSHK SGELSARLNA DVQEFKSSFK LCVSQGLRTF AQTIGCIGSL 

       250        260        270        280        290        300 
YFLSPTMTMY TVAVVPGIIL AGSAIGAGLR QLSRRAQGQS ATASAVSDEA LTNIRTIRAF 

       310        320        330        340        350        360 
AMEKLESRLF DNELDKARAM QEQLGVGIGL FQAGTNLFLN GMILSVLYGG SNLISKGEMT 

       370        380        390        400        410        420 
PGALMSFLVS AQTIQRSLSQ LSIIFGTAIK GWTAGGRVLQ FSRLEPSIPM DTGVCIPYHS 

       430        440        450        460        470        480 
LWGDIKFEDV SFSYPTRPGH TVFENLTLSI PAGQVVALCG PSGEGKSTIT HLLERFYEPK 

       490        500        510        520        530        540 
SGRVTLDGRD LRELNVEWLR GQVIGLISQE PVLFATSVEE NIRYGRPDAT DEEVREAARA 

       550        560        570        580        590        600 
AHVDEFVSRF PSGYSTVVGE RGAQLSGGQK QRIAIARAIL KNPPILILDE ATSALDSHSE 

       610        620        630        640        650        660 
HMVQEALNNV MKGRTVLIIA HRLSTIRSAQ MIYVIKDKKA LESGTHEQLM AKKGSLYRKL 


VEAHNVDS 

References and documentation are available.

Number of amino acids: 668

Molecular weight: 73352.84

Theoretical pI: 9.20

Amino acid composition: 
Ala (A)  58	  8.7%
Arg (R)  38	  5.7%
Asn (N)  19	  2.8%
Asp (D)  23	  3.4%
Cys (C)   9	  1.3%
Gln (Q)  29	  4.3%
Glu (E)  35	  5.2%
Gly (G)  51	  7.6%
His (H)  17	  2.5%
Ile (I)  48	  7.2%
Leu (L)  76	 11.4%
Lys (K)  33	  4.9%
Met (M)  18	  2.7%
Phe (F)  30	  4.5%
Pro (P)  22	  3.3%
Ser (S)  63	  9.4%
Thr (T)  39	  5.8%
Trp (W)   7	  1.0%
Tyr (Y)  14	  2.1%
Val (V)  39	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 71

Atomic composition:

Carbon      C	      3276
Hydrogen    H	      5252
Nitrogen    N	       904
Oxygen      O	       949
Sulfur      S	        27

Formula: C3276H5252N904O949S27
Total number of atoms: 10408

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    59860
Abs 0.1% (=1 g/l)   0.816, assuming all pairs of Cys residues form cystines


Ext. coefficient    59360
Abs 0.1% (=1 g/l)   0.809, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.38
This classifies the protein as stable.



Aliphatic index: 98.01

Grand average of hydropathicity (GRAVY): 0.046