ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSIMPVKNAL AQGRTLLMGR MPAVKFSTRM QLRNRTAVLW NRKFSTRLFV QQRRSSGEIV 

        70         80         90        100        110        120 
DRAKAAAANS GRKQVSMKWV VLTSFTIVLG TILLVSRNDS TEEDATEGKK GRRTRKIKIF 

       130        140        150        160        170        180 
NNNWLFFCYS TLPLNAMSRL WGQVNSLTLP IWVRPWGYRL YSFLFGVNLD EMEDPDLTHY 

       190        200        210        220        230        240 
ANLSEFFYRN IKPGTRPVAQ GEDVIASPSD GKILQVGIIN SETGEIEQVK GMTYSIKEFL 

       250        260        270        280        290        300 
GTHSHPLMSK SASSLDLTSD EEKHREFARV NRIQLAGSED TEQPLLNFKN EGDQSVREFK 

       310        320        330        340        350        360 
PSVSKNIHLL SQLSLNYFSN GFSCSEPHDT ELFFAVIYLA PGDYHHFHSP VDWVCKVRRH 

       370        380        390        400        410        420 
FPGDLFSVAP YFQRNFPNLF VLNERVALLG SWKYGFFSMT PVGATNVGSI KLNFDQEFVT 

       430        440        450        460        470        480 
NSKSDKHLEP HTCYQAVYEN ASKILGGMPL VKGEEMGGFE LGSTVVLCFE APTEFKFDVR 

       490        500 
VGDKVKMGQK LGIIGKNDLK 

References and documentation are available.

Number of amino acids: 500

Molecular weight: 56594.79

Theoretical pI: 9.18

Amino acid composition: 
Ala (A)  25	  5.0%
Arg (R)  29	  5.8%
Asn (N)  28	  5.6%
Asp (D)  21	  4.2%
Cys (C)   5	  1.0%
Gln (Q)  17	  3.4%
Glu (E)  30	  6.0%
Gly (G)  36	  7.2%
His (H)  12	  2.4%
Ile (I)  21	  4.2%
Leu (L)  48	  9.6%
Lys (K)  32	  6.4%
Met (M)  14	  2.8%
Phe (F)  33	  6.6%
Pro (P)  22	  4.4%
Ser (S)  43	  8.6%
Thr (T)  26	  5.2%
Trp (W)   8	  1.6%
Tyr (Y)  13	  2.6%
Val (V)  37	  7.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 61

Atomic composition:

Carbon      C	      2545
Hydrogen    H	      3958
Nitrogen    N	       696
Oxygen      O	       730
Sulfur      S	        19

Formula: C2545H3958N696O730S19
Total number of atoms: 7948

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    63620
Abs 0.1% (=1 g/l)   1.124, assuming all pairs of Cys residues form cystines


Ext. coefficient    63370
Abs 0.1% (=1 g/l)   1.120, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.67
This classifies the protein as stable.



Aliphatic index: 80.28

Grand average of hydropathicity (GRAVY): -0.294