ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSINICRDNH DPFYRYKMPP IQAKVEGRGN GIKTAVLNVA DISHALNRPA PYIVKYFGFE 

        70         80         90        100        110        120 
LGAQTSISVD KDRYLVNGVH EPAKLQDVLD GFINKFVLCG SCKNPETEII ITKDNDLVRD 

       130        140        150        160        170        180 
CKACGKRTPM DLRHKLSSFI LKNPPDSVSG SKKKKKAATA SANVRGGGLS ISDIAQGKSQ 

       190        200        210        220        230        240 
NAPSDGTGSS TPQHHDEDED ELSRQIKAAA STLEDIEVKD DEWAVDMSEE AIRARAKELE 

       250        260        270        280        290        300 
VNSELTQLDE YGEWILEQAG EDKENLPSDV ELYKKAAELD VLNDPKIGCV LAQCLFDEDI 

       310        320        330        340        350        360 
VNEIAEHNAF FTKILVTPEY EKNFMGGIER FLGLEHKDLI PLLPKILVQL YNNDIISEEE 

       370        380        390        400 
IMRFGTKSSK KFVPKEVSKK VRRAAKPFIT WLETAESDDD EEDDE 

References and documentation are available.

Number of amino acids: 405

Molecular weight: 45261.06

Theoretical pI: 4.94

Amino acid composition: 
Ala (A)  30	  7.4%
Arg (R)  16	  4.0%
Asn (N)  20	  4.9%
Asp (D)  35	  8.6%
Cys (C)   7	  1.7%
Gln (Q)  11	  2.7%
Glu (E)  39	  9.6%
Gly (G)  23	  5.7%
His (H)   8	  2.0%
Ile (I)  29	  7.2%
Leu (L)  33	  8.1%
Lys (K)  37	  9.1%
Met (M)   6	  1.5%
Phe (F)  14	  3.5%
Pro (P)  19	  4.7%
Ser (S)  27	  6.7%
Thr (T)  15	  3.7%
Trp (W)   3	  0.7%
Tyr (Y)   9	  2.2%
Val (V)  24	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 74
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      1991
Hydrogen    H	      3166
Nitrogen    N	       540
Oxygen      O	       636
Sulfur      S	        13

Formula: C1991H3166N540O636S13
Total number of atoms: 6346

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30285
Abs 0.1% (=1 g/l)   0.669, assuming all pairs of Cys residues form cystines


Ext. coefficient    29910
Abs 0.1% (=1 g/l)   0.661, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.05
This classifies the protein as unstable.



Aliphatic index: 84.30

Grand average of hydropathicity (GRAVY): -0.535