ProtParam
User-provided sequence:
10 20 30 40 50 60
MSIPETQKGV IFYESHGKLE YKDIPVPKPK ANELLINVKY SGVCHTDLHA WHGDWPLPVK
70 80 90 100 110 120
LPLVGGHEGA GVVVGMGENV KGWKIGDYAG IKWLNGSCMA CEYCELGNES NCPHADLSGY
130 140 150 160 170 180
THDGSFQQYA TADAVQAAHI PQGTDLAQVA PILCAGITVY KALKSANLMA GHWVAISGAA
190 200 210 220 230 240
GGLGSLAVQY AKAMGYRVLG IDGGEGKEEL FRSIGGEVFI DFTKEKDIVG AVLKATDGGA
250 260 270 280 290 300
HGVINVSVSE AAIEASTRYV RANGTTVLVG MPAGAKCCSD VFNQVVKSIS IVGSYVGNRA
310 320 330 340
DTREALDFFA RGLVKSPIKV VGLSTLPEIY EKMEKGQIVG RYVVDTSK
References and
documentation are available.
Number of amino acids: 348
Molecular weight: 36849.17
Theoretical pI: 6.21
Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 32
Atomic composition:
Carbon C 1644
Hydrogen H 2585
Nitrogen N 441
Oxygen O 490
Sulfur S 15
Formula: C
1644H
2585N
441O
490S
15
Total number of atoms: 5175
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 48860
Abs 0.1% (=1 g/l) 1.326, assuming all pairs of Cys residues form cystines
Ext. coefficient 48360
Abs 0.1% (=1 g/l) 1.312, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 20.71
This classifies the protein as stable.
Aliphatic index: 90.49
Grand average of hydropathicity (GRAVY): 0.030