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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSIQAFVFCG KGSNLAPFTQ PDFPFQTQNK DSTAATSGDK LNELVNSALD STVINEFMQH

70 80 90 100 110 120
STRLPKALLP IGNRPMIEYV LDWCDQADFK EISVVAPVDE IELIESGLTS FLSLRKQQFE

130 140 150 160 170 180
LIYKALSNSN HSHHLQDPKK INFIPSKANS TGESLQKELL PRINGDFVIL PCDFVTDIPP

190 200 210 220 230 240
QVLVDQFRNR DDNNLAMTIY YKNSLDSSID KKQQQKQKQQ QFFTVYSENE DSERQPILLD

250 260 270 280 290 300
VYSQRDVTKT KYLQIRSHLL WNYPNLTVST KLLNSFIYFC SFELCQLLKL GPQSMSRQAS

310 320 330 340 350 360
FKDPFTGNQQ QQNPPTTDDD EDRNHDDDDD YKPSATSIQP TYFKKKNDLI LDPINCNKSL

370 380 390 400 410 420
SKVFRDLSRR SWQHSKPREP IGIFILPNET LFIRANNLNA YMDANRFVLK IKSQTMFTKN

430 440 450 460 470 480
IQIQSAAIGA DAIVDPKCQI SAHSNVKMSV LGTQANIGSR CRVAGSLLFP GVHLGDEVIL

490 500 510 520 530 540
ENCIIGPMAK IGSKCKLSNC YIEGHYVVEP KNNFKGETLA NVYLDEDEED ELIYDDSVIA

550 560 570
GESEIAEETD SDDRSDEDSD DSEYTDEYEY EDDGLFER


References and documentation are available.

Number of amino acids: 578 Molecular weight: 65699.56 Theoretical pI: 4.78
Amino acid composition: 
Ala (A) 27 4.7% Arg (R) 21 3.6% Asn (N) 38 6.6% Asp (D) 52 9.0% Cys (C) 11 1.9% Gln (Q) 36 6.2% Glu (E) 36 6.2% Gly (G) 22 3.8% His (H) 10 1.7% Ile (I) 40 6.9% Leu (L) 52 9.0% Lys (K) 38 6.6% Met (M) 9 1.6% Phe (F) 29 5.0% Pro (P) 29 5.0% Ser (S) 52 9.0% Thr (T) 27 4.7% Trp (W) 3 0.5% Tyr (Y) 19 3.3% Val (V) 27 4.7% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 88 Total number of positively charged residues (Arg + Lys): 59 Atomic composition: Carbon C 2898 Hydrogen H 4514 Nitrogen N 776 Oxygen O 927 Sulfur S 20 Formula: C2898H4514N776O927S20 Total number of atoms: 9135 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 45435 Abs 0.1% (=1 g/l) 0.692, assuming all pairs of Cys residues form cystines Ext. coefficient 44810 Abs 0.1% (=1 g/l) 0.682, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 44.03 This classifies the protein as unstable. Aliphatic index: 80.29 Grand average of hydropathicity (GRAVY): -0.553