ProtParam
User-provided sequence:
10 20 30 40 50 60
MSIQAFVFCG KGSNLAPFTQ PDFPFQTQNK DSTAATSGDK LNELVNSALD STVINEFMQH
70 80 90 100 110 120
STRLPKALLP IGNRPMIEYV LDWCDQADFK EISVVAPVDE IELIESGLTS FLSLRKQQFE
130 140 150 160 170 180
LIYKALSNSN HSHHLQDPKK INFIPSKANS TGESLQKELL PRINGDFVIL PCDFVTDIPP
190 200 210 220 230 240
QVLVDQFRNR DDNNLAMTIY YKNSLDSSID KKQQQKQKQQ QFFTVYSENE DSERQPILLD
250 260 270 280 290 300
VYSQRDVTKT KYLQIRSHLL WNYPNLTVST KLLNSFIYFC SFELCQLLKL GPQSMSRQAS
310 320 330 340 350 360
FKDPFTGNQQ QQNPPTTDDD EDRNHDDDDD YKPSATSIQP TYFKKKNDLI LDPINCNKSL
370 380 390 400 410 420
SKVFRDLSRR SWQHSKPREP IGIFILPNET LFIRANNLNA YMDANRFVLK IKSQTMFTKN
430 440 450 460 470 480
IQIQSAAIGA DAIVDPKCQI SAHSNVKMSV LGTQANIGSR CRVAGSLLFP GVHLGDEVIL
490 500 510 520 530 540
ENCIIGPMAK IGSKCKLSNC YIEGHYVVEP KNNFKGETLA NVYLDEDEED ELIYDDSVIA
550 560 570
GESEIAEETD SDDRSDEDSD DSEYTDEYEY EDDGLFER
References and
documentation are available.
Number of amino acids: 578
Molecular weight: 65699.56
Theoretical pI: 4.78
Total number of negatively charged residues (Asp + Glu): 88
Total number of positively charged residues (Arg + Lys): 59
Atomic composition:
Carbon C 2898
Hydrogen H 4514
Nitrogen N 776
Oxygen O 927
Sulfur S 20
Formula: C
2898H
4514N
776O
927S
20
Total number of atoms: 9135
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 45435
Abs 0.1% (=1 g/l) 0.692, assuming all pairs of Cys residues form cystines
Ext. coefficient 44810
Abs 0.1% (=1 g/l) 0.682, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.03
This classifies the protein as unstable.
Aliphatic index: 80.29
Grand average of hydropathicity (GRAVY): -0.553