ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSIQTSDPNE TSDLKSLSLI AAHSHITGLG LDENLQPRPT SEGMVGQLQA RRAAGVILKM 

        70         80         90        100        110        120 
VQNGTIAGRA VLVAGPPSTG KTALAMGVSQ SLGKDVPFTA IAGSEIFSLE LSKTEALTQA 

       130        140        150        160        170        180 
FRKSIGIKIK EETELIEGEV VEIQIDRSIT GGHKQGKLTI KTTDMETIYE LGNKMIDGLT 

       190        200        210        220        230        240 
KEKVLAGDVI SIDKASGKIT KLGRSFARSR DYDAMGADTR FVQCPEGELQ KRKTVVHTVS 

       250        260        270        280        290        300 
LHEIDVINSR TQGFLALFTG DTGEIRSEVR DQINTKVAEW KEEGKAEIVP GVLFIDEVHM 

       310        320        330        340        350        360 
LDIECFSFIN RALEDEFAPI VMMATNRGVS KTRGTNYKSP HGLPLDLLDR SIIITTKSYN 

       370        380        390        400        410        420 
EQEIKTILSI RAQEEEVELS SDALDLLTKT GVETSLRYSS NLISVAQQIA MKRKNNTVEV 

       430        440        450        460        470 
EDVKRAYLLF LDSARSVKYV QENESQYIDD QGNVQISIAK SADPDAMDTT E 

References and documentation are available.

Number of amino acids: 471

Molecular weight: 51611.64

Theoretical pI: 5.20

Amino acid composition: 
Ala (A)  34	  7.2%
Arg (R)  23	  4.9%
Asn (N)  15	  3.2%
Asp (D)  29	  6.2%
Cys (C)   2	  0.4%
Gln (Q)  21	  4.5%
Glu (E)  40	  8.5%
Gly (G)  34	  7.2%
His (H)   7	  1.5%
Ile (I)  40	  8.5%
Leu (L)  41	  8.7%
Lys (K)  32	  6.8%
Met (M)  12	  2.5%
Phe (F)  12	  2.5%
Pro (P)  12	  2.5%
Ser (S)  39	  8.3%
Thr (T)  37	  7.9%
Trp (W)   1	  0.2%
Tyr (Y)   8	  1.7%
Val (V)  32	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 69
Total number of positively charged residues (Arg + Lys): 55

Atomic composition:

Carbon      C	      2251
Hydrogen    H	      3691
Nitrogen    N	       623
Oxygen      O	       730
Sulfur      S	        14

Formula: C2251H3691N623O730S14
Total number of atoms: 7309

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    17545
Abs 0.1% (=1 g/l)   0.340, assuming all pairs of Cys residues form cystines


Ext. coefficient    17420
Abs 0.1% (=1 g/l)   0.338, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.80
This classifies the protein as stable.



Aliphatic index: 93.99

Grand average of hydropathicity (GRAVY): -0.269