ProtParam
User-provided sequence:
10 20 30 40 50 60
MSISLLGRIV SQQFSGIRAA EPGRSLYLPF TLLLKQPGAY KVSLHRYVHS TQTKSHLSFL
70 80 90 100 110 120
MNNNDITPFQ KFTVKVLKEQ CKSRGLKLSG RKSDLLQRLI THDSCSNKKS SVKINEPKKK
130 140 150 160 170 180
RILINDPIKI TKKLVSDKTF RTIEKNISSL QNTPVIETPC DVHSHLQPRD RIFLLGFFML
190
SCLWWNLEPQ ESKPTIDH
References and
documentation are available.
Number of amino acids: 198
Molecular weight: 22741.59
Theoretical pI: 10.02
Total number of negatively charged residues (Asp + Glu): 15
Total number of positively charged residues (Arg + Lys): 32
Atomic composition:
Carbon C 1021
Hydrogen H 1661
Nitrogen N 287
Oxygen O 285
Sulfur S 7
Formula: C
1021H
1661N
287O
285S
7
Total number of atoms: 3261
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 15720
Abs 0.1% (=1 g/l) 0.691, assuming all pairs of Cys residues form cystines
Ext. coefficient 15470
Abs 0.1% (=1 g/l) 0.680, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.96
This classifies the protein as unstable.
Aliphatic index: 93.48
Grand average of hydropathicity (GRAVY): -0.395