ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSISTSLNSA SIHLSSMDTH PQLHSLTRQP HSSSTAMSKN EAQESSPSLP ASSSSSTSAS 

        70         80         90        100        110        120 
ASASSKNSSK NPSSWDPQDD LLLRHLKEVK KMGWKDISQY FPNRTPNACQ FRWRRLKSGN 

       130        140        150        160        170        180 
LKSNKTALID INTYTGPLKI THGDETANAQ QKPSKKVEEN VLTEDTAEFT TTSSIPIPSR 

       190        200        210        220        230        240 
KTSLPSFHAS MSFSQSPSNV TPTTIVSNAA SSMPFAPPTL PAALPHHPHQ HLHHHPHHKT 

       250        260        270        280        290        300 
LKPRSNSHSF TNSLNQDPIV RSNDEEKYGF IPKVFVRSRR SSFAYPQQVA ITTTPSSPNS 

       310        320        330        340        350        360 
SHVLLSSKSR RGSLANWSRR SSFNVSSNNT SRRSSMILAP NSVSNIFNVN NSGSNTASTS 

       370        380        390        400        410        420 
NTNSRRESVI KKEFQQRLNN LSNSGGPTSN NGPIFPNSYT FMDLPHSSSV SSSSTLHKSK 

       430        440        450        460        470        480 
RGSFSGHSMK SSCNPTNLWS KDEDALLMEN KKRNLSVMEL SILLPQRTEV EIQWRLNALS 

       490        500        510        520        530        540 
SDADMLSPTH SPQKTLSKKT CPRMFKSGST TDDDKGSDKE DVMGDGSNDD DEDNVDPLHR 

       550        560        570        580        590        600 
AKQSSNKTVF SSSSSNISSK DVSPDPIFSP DPADDSSNTS DAGSRCTITS DTSSSAATMN 

       610        620        630        640        650        660 
RTPNSKNPQD IALLNNFRSE AITPRPKPSS TTTSITTETT NNMINHSSST TTTTNNSPLP 

       670 
SINTIFKDML 

References and documentation are available.

Number of amino acids: 670

Molecular weight: 73048.40

Theoretical pI: 9.50

Amino acid composition: 
Ala (A)  33	  4.9%
Arg (R)  31	  4.6%
Asn (N)  57	  8.5%
Asp (D)  35	  5.2%
Cys (C)   4	  0.6%
Gln (Q)  20	  3.0%
Glu (E)  21	  3.1%
Gly (G)  17	  2.5%
His (H)  24	  3.6%
Ile (I)  27	  4.0%
Leu (L)  44	  6.6%
Lys (K)  40	  6.0%
Met (M)  17	  2.5%
Phe (F)  21	  3.1%
Pro (P)  50	  7.5%
Ser (S) 136	 20.3%
Thr (T)  61	  9.1%
Trp (W)   6	  0.9%
Tyr (Y)   5	  0.7%
Val (V)  21	  3.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 71

Atomic composition:

Carbon      C	      3106
Hydrogen    H	      4956
Nitrogen    N	       934
Oxygen      O	      1062
Sulfur      S	        21

Formula: C3106H4956N934O1062S21
Total number of atoms: 10079

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40700
Abs 0.1% (=1 g/l)   0.557, assuming all pairs of Cys residues form cystines


Ext. coefficient    40450
Abs 0.1% (=1 g/l)   0.554, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 60.25
This classifies the protein as unstable.



Aliphatic index: 55.34

Grand average of hydropathicity (GRAVY): -0.822