ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSITKVHART VYDSRGNPTV EVEITTENGL FRAIVPSGAS TGIHEAVELR DGNKSEWMGK 

        70         80         90        100        110        120 
GVTKAVSNVN SIIGPALIKS DLCVTNQKGI DELMISLDGT SNKSRLGANA ILGVSLCVAR 

       130        140        150        160        170        180 
AAAAQKGITL YKYIAELADA RQDPFVIPVP FFNVLNGGAH AGGSLAMQEF KIAPVGAQSF 

       190        200        210        220        230        240 
AEAMRMGSEV YHHLKILAKE QYGPSAGNVG DEGGVAPDID TAEDALDMIV KAINICGYEG 

       250        260        270        280        290        300 
RVKVGIDSAP SVFYKDGKYD LNFKEPNSDP SHWLSPAQLA EYYHSLLKKY PIISLEDPYA 

       310        320        330        340        350        360 
EDDWSSWSAF LKTVNVQIIA DDLTCTNKTR IARAIEEKCA NTLLLKLNQI GTLTESIEAA 

       370        380        390        400        410        420 
NQAFDAGWGV MISHRSGETE DPFIADLVVG LRCGQIKSGA LSRSERLAKY NELLRIEEEL 

       430 
GDDCIYAGHR FHDGNKL 

References and documentation are available.

Number of amino acids: 437

Molecular weight: 47312.61

Theoretical pI: 5.26

Amino acid composition: 
Ala (A)  46	 10.5%
Arg (R)  17	  3.9%
Asn (N)  21	  4.8%
Asp (D)  27	  6.2%
Cys (C)   7	  1.6%
Gln (Q)  11	  2.5%
Glu (E)  30	  6.9%
Gly (G)  38	  8.7%
His (H)  10	  2.3%
Ile (I)  33	  7.6%
Leu (L)  37	  8.5%
Lys (K)  26	  5.9%
Met (M)   8	  1.8%
Phe (F)  12	  2.7%
Pro (P)  16	  3.7%
Ser (S)  32	  7.3%
Thr (T)  19	  4.3%
Trp (W)   5	  1.1%
Tyr (Y)  14	  3.2%
Val (V)  28	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 57
Total number of positively charged residues (Arg + Lys): 43

Atomic composition:

Carbon      C	      2091
Hydrogen    H	      3309
Nitrogen    N	       571
Oxygen      O	       649
Sulfur      S	        15

Formula: C2091H3309N571O649S15
Total number of atoms: 6635

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    48735
Abs 0.1% (=1 g/l)   1.030, assuming all pairs of Cys residues form cystines


Ext. coefficient    48360
Abs 0.1% (=1 g/l)   1.022, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.71
This classifies the protein as stable.



Aliphatic index: 91.58

Grand average of hydropathicity (GRAVY): -0.156