ProtParam
User-provided sequence:
10 20 30 40 50 60
MSITKVHART VYDSRGNPTV EVEITTENGL FRAIVPSGAS TGIHEAVELR DGNKSEWMGK
70 80 90 100 110 120
GVTKAVSNVN SIIGPALIKS ELCVTNQKGI DELMISLDGT SNKSRLGANA ILGVSLCVAR
130 140 150 160 170 180
AAAAQKGITL YKYIAELADA RQDPFVIPVP FFNVLNGGAH AGGSLAMQEF KIAPVGAQSF
190 200 210 220 230 240
AEAMRMGSEV YHHLKILAKE QYGPSAGNVG DEGGVAPDID TAEDALDMIV EAINICGYEG
250 260 270 280 290 300
RVKVGIDSAP SVFYKDGKYD LNFKEPNSDP SHWLSPAQLA EYYHSLLKKY PIISLEDPYA
310 320 330 340 350 360
EDDWSSWSAF LKTVNVQIIA DDLTCTNKTR IARAIEEKCA NTLLLKLNQI GTLTESIEAA
370 380 390 400 410 420
NQAFDAGWGV MISHRSGETE DPFIADLVVG LRCGQIKSGA LSRSERLAKY NELLRIEEEL
430
GDDCIYAGHR FHDGNKL
References and
documentation are available.
Number of amino acids: 437
Molecular weight: 47327.58
Theoretical pI: 5.15
Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 42
Atomic composition:
Carbon C 2091
Hydrogen H 3306
Nitrogen N 570
Oxygen O 651
Sulfur S 15
Formula: C
2091H
3306N
570O
651S
15
Total number of atoms: 6633
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 48735
Abs 0.1% (=1 g/l) 1.030, assuming all pairs of Cys residues form cystines
Ext. coefficient 48360
Abs 0.1% (=1 g/l) 1.022, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.21
This classifies the protein as stable.
Aliphatic index: 91.58
Grand average of hydropathicity (GRAVY): -0.155