ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSITSEELNY LIWRYCQEMG HEVSALALQD ETRVLEFDEK YKEHIPLGTL VNLVQRGILY 

        70         80         90        100        110        120 
TESELMVDSK GDISALNEHH LSEDFNLVQA LQIDKEKFPE ISSEGRFTLE TNSESNKAGE 

       130        140        150        160        170        180 
DGASTVERET QEDDTNSIDS SDDLDGFVKI LKEIVKLDNI VSSTWNPLDE SILAYGEKNS 

       190        200        210        220        230        240 
VARLARIVET DQEGKKYWKL TIIAELRHPF ALSASSGKTT NQVTCLAWSH DGNSIVTGVE 

       250        260        270        280        290        300 
NGELRLWNKT GALLNVLNFH RAPIVSVKWN KDGTHIISMD VENVTILWNV ISGTVMQHFE 

       310        320        330        340        350        360 
LKETGGSSIN AENHSGDGSL GVDVEWVDDD KFVIPGPKGA IFVYQITEKT PTGKLIGHHG 

       370        380        390        400        410        420 
PISVLEFNDT NKLLLSASDD GTLRIWHGGN GNSQNCFYGH SQSIVSASWV GDDKVISCSM 

       430        440        450        460        470        480 
DGSVRLWSLK QNTLLALSIV DGVPIFAGRI SQDGQKYAVA FMDGQVNVYD LKKLNSKSRS 

       490        500        510        520        530 
LYGNRDGILN PLPIPLYASY QSSQDNDYIF DLSWNCAGNK ISVAYSLQEG SVVAI 

References and documentation are available.

Number of amino acids: 535

Molecular weight: 59161.18

Theoretical pI: 4.81

Amino acid composition: 
Ala (A)  27	  5.0%
Arg (R)  15	  2.8%
Asn (N)  34	  6.4%
Asp (D)  38	  7.1%
Cys (C)   5	  0.9%
Gln (Q)  19	  3.6%
Glu (E)  37	  6.9%
Gly (G)  42	  7.9%
His (H)  14	  2.6%
Ile (I)  38	  7.1%
Leu (L)  52	  9.7%
Lys (K)  30	  5.6%
Met (M)   7	  1.3%
Phe (F)  15	  2.8%
Pro (P)  13	  2.4%
Ser (S)  55	 10.3%
Thr (T)  27	  5.0%
Trp (W)  12	  2.2%
Tyr (Y)  15	  2.8%
Val (V)  40	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 75
Total number of positively charged residues (Arg + Lys): 45

Atomic composition:

Carbon      C	      2617
Hydrogen    H	      4089
Nitrogen    N	       703
Oxygen      O	       836
Sulfur      S	        12

Formula: C2617H4089N703O836S12
Total number of atoms: 8257

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    88600
Abs 0.1% (=1 g/l)   1.498, assuming all pairs of Cys residues form cystines


Ext. coefficient    88350
Abs 0.1% (=1 g/l)   1.493, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 28.80
This classifies the protein as stable.



Aliphatic index: 92.34

Grand average of hydropathicity (GRAVY): -0.290