ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSIVGRNAIL NLRISLCPLF MGKRSFVSSP VSNSAKAVKF LKAQRRKQKN EAKQATLKAS 

        70         80         90        100        110        120 
TDKVDPVLGR ADTPFITRIM AELKEPLVLS KGYNIEEVDK FLAAIESAKR ERAELSGLNT 

       130        140        150        160        170        180 
EVVGIEDIEK LEDRREAILR ILSMRNSENK NAIKMAVELA RKEFERFPGD TGSSEVQAAC 

       190        200        210        220        230        240 
MTVRIQNMAN HIKEHRKDFA NTRNLRILVQ QRQAILRYLK RDNPEKYYWT IQKLGLNDAA 

       250        260        270        280 
ITDEFNMDRR YMQDYEFFGD KILIRDSKKV ANQKRKEIRK QKRATF 

References and documentation are available.

Number of amino acids: 286

Molecular weight: 33057.27

Theoretical pI: 9.92

Amino acid composition: 
Ala (A)  26	  9.1%
Arg (R)  28	  9.8%
Asn (N)  17	  5.9%
Asp (D)  15	  5.2%
Cys (C)   2	  0.7%
Gln (Q)  12	  4.2%
Glu (E)  23	  8.0%
Gly (G)  10	  3.5%
His (H)   2	  0.7%
Ile (I)  21	  7.3%
Leu (L)  24	  8.4%
Lys (K)  29	 10.1%
Met (M)   9	  3.1%
Phe (F)  12	  4.2%
Pro (P)   7	  2.4%
Ser (S)  16	  5.6%
Thr (T)  11	  3.8%
Trp (W)   1	  0.3%
Tyr (Y)   6	  2.1%
Val (V)  15	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 57

Atomic composition:

Carbon      C	      1451
Hydrogen    H	      2393
Nitrogen    N	       433
Oxygen      O	       425
Sulfur      S	        11

Formula: C1451H2393N433O425S11
Total number of atoms: 4713

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    14565
Abs 0.1% (=1 g/l)   0.441, assuming all pairs of Cys residues form cystines


Ext. coefficient    14440
Abs 0.1% (=1 g/l)   0.437, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 55.54
This classifies the protein as unstable.



Aliphatic index: 85.66

Grand average of hydropathicity (GRAVY): -0.606