ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSIVSLLGIK VLNNPAKFTD PYEFEITFEC LESLKHDLEW KLTYVGSSRS LDHDQELDSI 

        70         80         90        100        110        120 
LVGPVPVGVN KFVFSADPPS AELIPASELV SVTVILLSCS YDGREFVRVG YYVNNEYDEE 

       130        140        150        160        170        180 
ELRENPPAKV QVDHIVRNIL AEKPRVTRFN IVWDNENEGD LYPPEQPGVD DEEEEDDEEE 

       190        200        210        220        230        240 
DDDEDDEDDE DDDQEDGEGE AEEAAEEEEE EEEKTEDNET NLEEEEEDIE NSDGDEEEGE 

       250        260        270 
EEVGSVDKNE DGNDKKRRKI EGGSTDIEST PKDAARSTN 

References and documentation are available.

Number of amino acids: 279

Molecular weight: 31603.41

Theoretical pI: 3.88

Amino acid composition: 
Ala (A)  11	  3.9%
Arg (R)  10	  3.6%
Asn (N)  16	  5.7%
Asp (D)  35	 12.5%
Cys (C)   2	  0.7%
Gln (Q)   4	  1.4%
Glu (E)  58	 20.8%
Gly (G)  16	  5.7%
His (H)   3	  1.1%
Ile (I)  12	  4.3%
Leu (L)  18	  6.5%
Lys (K)  13	  4.7%
Met (M)   1	  0.4%
Phe (F)   7	  2.5%
Pro (P)  14	  5.0%
Ser (S)  18	  6.5%
Thr (T)  10	  3.6%
Trp (W)   2	  0.7%
Tyr (Y)   7	  2.5%
Val (V)  22	  7.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 93
Total number of positively charged residues (Arg + Lys): 23

Atomic composition:

Carbon      C	      1348
Hydrogen    H	      2062
Nitrogen    N	       350
Oxygen      O	       521
Sulfur      S	         3

Formula: C1348H2062N350O521S3
Total number of atoms: 4284

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    21555
Abs 0.1% (=1 g/l)   0.682, assuming all pairs of Cys residues form cystines


Ext. coefficient    21430
Abs 0.1% (=1 g/l)   0.678, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 75.08
This classifies the protein as unstable.



Aliphatic index: 68.75

Grand average of hydropathicity (GRAVY): -1.078