ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSIVSRTVTI TISVKKLITY IELRYGMESS TCPFCQSGTL ISLAAVFFCH SGMEALLSIV 

        70         80         90        100        110        120 
CSLAFFHAGT ALSSLLASLP FSFSLSLSSC MPILARISDA DGIVSMPGIP LGDMENNLLS 

       130        140        150        160        170        180 
CALPDSIRLF IKLFRDSISF WILFNSCMPS LSETNLSIVF CILTTSSLTI LNSSSIFSLL 

       190        200        210        220 
LVVCTSACFS SAIVSLSSFN VSLSFFLNSA CSASMALRTV SILEN 

References and documentation are available.

Number of amino acids: 225

Molecular weight: 24082.36

Theoretical pI: 5.56

Amino acid composition: 
Ala (A)  15	  6.7%
Arg (R)   6	  2.7%
Asn (N)   8	  3.6%
Asp (D)   5	  2.2%
Cys (C)  11	  4.9%
Gln (Q)   1	  0.4%
Glu (E)   6	  2.7%
Gly (G)   7	  3.1%
His (H)   2	  0.9%
Ile (I)  21	  9.3%
Leu (L)  34	 15.1%
Lys (K)   3	  1.3%
Met (M)   8	  3.6%
Phe (F)  17	  7.6%
Pro (P)   7	  3.1%
Ser (S)  46	 20.4%
Thr (T)  13	  5.8%
Trp (W)   1	  0.4%
Tyr (Y)   2	  0.9%
Val (V)  12	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 11
Total number of positively charged residues (Arg + Lys): 9

Atomic composition:

Carbon      C	      1082
Hydrogen    H	      1734
Nitrogen    N	       260
Oxygen      O	       318
Sulfur      S	        19

Formula: C1082H1734N260O318S19
Total number of atoms: 3413

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     9105
Abs 0.1% (=1 g/l)   0.378, assuming all pairs of Cys residues form cystines


Ext. coefficient     8480
Abs 0.1% (=1 g/l)   0.352, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.94
This classifies the protein as unstable.



Aliphatic index: 117.47

Grand average of hydropathicity (GRAVY): 0.946