ProtParam
User-provided sequence:
10 20 30 40 50 60
MSIVYNKTPL LRQFFPGKAS AQFFLKYECL QPSGSFKSRG IGNLIMKSAI RIQKDGKRSP
70 80 90 100 110 120
QVFASSGGNA GFAAATACQR LSLPCTVVVP TATKKRMVDK IRNTGAQVIV SGAYWKEADT
130 140 150 160 170 180
FLKTNVMNKI DSQVIEPIYV HPFDNPDIWE GHSSMIDEIV QDLKSQHISV NKVKGIVCSV
190 200 210 220 230 240
GGGGLYNGII QGLERYGLAD RIPIVGVETN GCHVFNTSLK IGQPVQFKKI TSIATSLGTA
250 260 270 280 290 300
VISNQTFEYA RKYNTRSVVI EDKDVIETCL KYTHQFNMVI EPACGAALHL GYNTKILENA
310 320 330 340 350 360
LGSKLAADDI VIIIACGGSS NTIKDLEEAL DSMRKKDTPV IEVADNFIFP EKNIVNLKSA
References and
documentation are available.
Number of amino acids: 360
Molecular weight: 39301.36
Theoretical pI: 8.95
Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 41
Atomic composition:
Carbon C 1752
Hydrogen H 2812
Nitrogen N 474
Oxygen O 519
Sulfur S 15
Formula: C
1752H
2812N
474O
519S
15
Total number of atoms: 5572
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 26400
Abs 0.1% (=1 g/l) 0.672, assuming all pairs of Cys residues form cystines
Ext. coefficient 25900
Abs 0.1% (=1 g/l) 0.659, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.80
This classifies the protein as stable.
Aliphatic index: 93.69
Grand average of hydropathicity (GRAVY): -0.049