ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSKATCSFEG CHSAVITIND DNIINLPEQV HSEFKLLENR TMRDATPSES NFLVVPDVWD 

        70         80         90        100        110        120 
FDNVGVSREI PSSILGDLSD KSDFVFEYGN SSWKIKKCLK YLICADCDKG PIGIICKVQD 

       130        140 
QTKNEERVLH LLSLRSLQIM GRN 

References and documentation are available.

Number of amino acids: 143

Molecular weight: 16126.38

Theoretical pI: 5.15

Amino acid composition: 
Ala (A)   4	  2.8%
Arg (R)   6	  4.2%
Asn (N)   9	  6.3%
Asp (D)  12	  8.4%
Cys (C)   6	  4.2%
Gln (Q)   4	  2.8%
Glu (E)   9	  6.3%
Gly (G)   7	  4.9%
His (H)   3	  2.1%
Ile (I)  12	  8.4%
Leu (L)  13	  9.1%
Lys (K)  10	  7.0%
Met (M)   3	  2.1%
Phe (F)   6	  4.2%
Pro (P)   5	  3.5%
Ser (S)  15	 10.5%
Thr (T)   5	  3.5%
Trp (W)   2	  1.4%
Tyr (Y)   2	  1.4%
Val (V)  10	  7.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 21
Total number of positively charged residues (Arg + Lys): 16

Atomic composition:

Carbon      C	       706
Hydrogen    H	      1124
Nitrogen    N	       192
Oxygen      O	       221
Sulfur      S	         9

Formula: C706H1124N192O221S9
Total number of atoms: 2252

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    14355
Abs 0.1% (=1 g/l)   0.890, assuming all pairs of Cys residues form cystines


Ext. coefficient    13980
Abs 0.1% (=1 g/l)   0.867, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.17
This classifies the protein as stable.



Aliphatic index: 91.26

Grand average of hydropathicity (GRAVY): -0.246