ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSKATYKERA ATHPSPVAAK LFNIMHEKQT NLCASLDVRT TKELLELVEA LGPKICLLKT 

        70         80         90        100        110        120 
HVDILTDFSM EGTVKPLKAL SAKYNFLLFE DRKFADIGNT VKLQYSAGVY RIAEWADITN 

       130        140        150        160        170        180 
AHGVVGPGIV SGLKQAAEEV TKEPRGLLML AELSCKGSLA TGEYTKGTVD IAKSDKDFVI 

       190        200        210        220        230        240 
GFIAQRDMGG RDEGYDWLIM TPGVGLDDKG DALGQQYRTV DDVVSTGSDI IIVGRGLFAK 

       250        260 
GRDAKVEGER YRKAGWEAYL RRCGQQN 

References and documentation are available.

Number of amino acids: 267

Molecular weight: 29239.51

Theoretical pI: 6.91

Amino acid composition: 
Ala (A)  25	  9.4%
Arg (R)  14	  5.2%
Asn (N)   6	  2.2%
Asp (D)  19	  7.1%
Cys (C)   4	  1.5%
Gln (Q)   8	  3.0%
Glu (E)  17	  6.4%
Gly (G)  27	 10.1%
His (H)   4	  1.5%
Ile (I)  14	  5.2%
Leu (L)  26	  9.7%
Lys (K)  22	  8.2%
Met (M)   6	  2.2%
Phe (F)   8	  3.0%
Pro (P)   7	  2.6%
Ser (S)  12	  4.5%
Thr (T)  17	  6.4%
Trp (W)   3	  1.1%
Tyr (Y)   9	  3.4%
Val (V)  19	  7.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 36

Atomic composition:

Carbon      C	      1296
Hydrogen    H	      2078
Nitrogen    N	       356
Oxygen      O	       392
Sulfur      S	        10

Formula: C1296H2078N356O392S10
Total number of atoms: 4132

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30160
Abs 0.1% (=1 g/l)   1.031, assuming all pairs of Cys residues form cystines


Ext. coefficient    29910
Abs 0.1% (=1 g/l)   1.023, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 12.57
This classifies the protein as stable.



Aliphatic index: 88.43

Grand average of hydropathicity (GRAVY): -0.240