ProtParam
User-provided sequence:
10 20 30 40 50 60
MSKAVGIDLG TTYSCVAHFS NDRVDIIAND QGNRTTPSFV GFTDTERLIG DAAKNQAAMN
70 80 90 100 110 120
PANTVFDAKR LIGRNFNDPE VQGDMKHFPF KLIDVDGKPQ IQVEFKGETK NFTPEQISSM
130 140 150 160 170 180
VLGKMKETAE SYLGAKVNDA VVTVPAYFND SQRQATKDAG TIAGLNVLRI INEPTAAAIA
190 200 210 220 230 240
YGLDKKGKEE HVLIFDLGGG TFDVSLLSIE DGIFEVKATA GDTHLGGEDF DNRLVNHFIQ
250 260 270 280 290 300
EFKRKNKKDL STNQRALRRL RTACERAKRT LSSSAQTSVE IDSLFEGIDF YTSITRARFE
310 320 330 340 350 360
ELCADLFRST LDPVEKVLRD AKLDKSQVDE IVLVGGSTRI PKVQKLVTDY FNGKEPNRSI
370 380 390 400 410 420
NPDEAVAYGA AVQAAILTGD ESSKTQDLLL LDVAPLSLGI ETAGGVMTKL IPRNSTIPTK
430 440 450 460 470 480
KSEVFSTYAD NQPGVLIQVF EGERAKTKDN NLLGKFELSG IPPAPRGVPQ IEVTFDVDSN
490 500 510 520 530 540
GILNVSAVEK GTGKSNKITI TNDKGRLSKE DIEKMVAEAE KFKEEDEKES QRIASKNQLE
550 560 570 580 590 600
SIAYSLKNTI SEAGDKLEQA DKDAVTKKAE ETIAWLDSNT TATKEEFDDQ LKELQEVANP
610 620 630
IMSKLYQAGG APEGAAPGGF PGGAPPAPEA EGPTVEEVD
References and
documentation are available.
Number of amino acids: 639
Molecular weight: 69470.01
Theoretical pI: 4.94
Total number of negatively charged residues (Asp + Glu): 101
Total number of positively charged residues (Arg + Lys): 78
Atomic composition:
Carbon C 3048
Hydrogen H 4888
Nitrogen N 836
Oxygen O 992
Sulfur S 11
Formula: C
3048H
4888N
836O
992S
11
Total number of atoms: 9775
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 20525
Abs 0.1% (=1 g/l) 0.295, assuming all pairs of Cys residues form cystines
Ext. coefficient 20400
Abs 0.1% (=1 g/l) 0.294, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.41
This classifies the protein as stable.
Aliphatic index: 81.24
Grand average of hydropathicity (GRAVY): -0.446