ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSKDKDIKVT PGTCELVEQI LALLSRYLSS YIHVLNKFIS HLRRVATLRF ERTTLIKFVK 

        70         80         90        100        110        120 
KLRFYNDCVL SYNASEFINE GKNELDPEAD SFDKVILPIA SMFVKCVETF DLLNYYLTQS 

       130        140        150        160        170        180 
LQKEILSKTL NEDLTLTAES ILAIDDTYNH FVKFSQWMIE SLRIGSNLLD LEVVQFAIKC 

       190        200        210        220        230        240 
ADEDGTNIGE TDNIFLQEIL PVNSEEEFQT LSAAWHSILD GKLSALDEEF DVVATKWHDK 


FGKLKN 

References and documentation are available.

Number of amino acids: 246

Molecular weight: 28251.31

Theoretical pI: 4.86

Amino acid composition: 
Ala (A)  13	  5.3%
Arg (R)   7	  2.8%
Asn (N)  13	  5.3%
Asp (D)  18	  7.3%
Cys (C)   4	  1.6%
Gln (Q)   7	  2.8%
Glu (E)  21	  8.5%
Gly (G)   7	  2.8%
His (H)   5	  2.0%
Ile (I)  18	  7.3%
Leu (L)  33	 13.4%
Lys (K)  19	  7.7%
Met (M)   3	  1.2%
Phe (F)  15	  6.1%
Pro (P)   4	  1.6%
Ser (S)  19	  7.7%
Thr (T)  15	  6.1%
Trp (W)   3	  1.2%
Tyr (Y)   7	  2.8%
Val (V)  15	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 26

Atomic composition:

Carbon      C	      1279
Hydrogen    H	      2005
Nitrogen    N	       319
Oxygen      O	       386
Sulfur      S	         7

Formula: C1279H2005N319O386S7
Total number of atoms: 3996

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    27180
Abs 0.1% (=1 g/l)   0.962, assuming all pairs of Cys residues form cystines


Ext. coefficient    26930
Abs 0.1% (=1 g/l)   0.953, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.14
This classifies the protein as unstable.



Aliphatic index: 103.82

Grand average of hydropathicity (GRAVY): -0.099