ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSKEDFVIKP EAAGASTDTS EWPLLLKNFD KLLVRSGHYT PIPAGSSPLK RDLKSYISSG 

        70         80         90        100        110        120 
VINLDKPSNP SSHEVVAWIK RILRCEKTGH SGTLDPKVTG CLIVCIDRAT RLVKSQQGAG 

       130        140        150        160        170        180 
KEYVCIVRLH DALKDEKDLG RSLENLTGAL FQRPPLISAV KRQLRVRTIY ESNLIEFDNK 

       190        200        210        220        230        240 
RNLGVFWASC EAGTYMRTLC VHLGMLLGVG GHMQELRRVR SGALSENDNM VTLHDVMDAQ 

       250        260        270        280        290        300 
WVYDNTRDES YLRSIIQPLE TLLVGYKRIV VKDSAVNAVC YGAKLMIPGL LRYEEGIELY 

       310        320        330        340        350        360 
DEIVLITTKG EAIAVAIAQM STVDLASCDH GVVASVKRCI MERDLYPRRW GLGPVAQKKK 

       370        380        390        400        410        420 
QMKADGKLDK YGRVNENTPE QWKKEYVPLD NAEQSTSSSQ ETKETEEEPK KAKEDSLIKE 

       430        440        450        460        470        480 
VETEKEEVKE DDSKKEKKEK KDKKEKKEKK EKKDKKEKKE KKEKKRKSED GDSEEKKSKK 


SKK 

References and documentation are available.

Number of amino acids: 483

Molecular weight: 54704.83

Theoretical pI: 9.02

Amino acid composition: 
Ala (A)  27	  5.6%
Arg (R)  27	  5.6%
Asn (N)  14	  2.9%
Asp (D)  31	  6.4%
Cys (C)   9	  1.9%
Gln (Q)  13	  2.7%
Glu (E)  48	  9.9%
Gly (G)  29	  6.0%
His (H)   8	  1.7%
Ile (I)  23	  4.8%
Leu (L)  44	  9.1%
Lys (K)  65	 13.5%
Met (M)  10	  2.1%
Phe (F)   5	  1.0%
Pro (P)  17	  3.5%
Ser (S)  36	  7.5%
Thr (T)  22	  4.6%
Trp (W)   6	  1.2%
Tyr (Y)  14	  2.9%
Val (V)  35	  7.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 79
Total number of positively charged residues (Arg + Lys): 92

Atomic composition:

Carbon      C	      2396
Hydrogen    H	      3934
Nitrogen    N	       678
Oxygen      O	       741
Sulfur      S	        19

Formula: C2396H3934N678O741S19
Total number of atoms: 7768

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    54360
Abs 0.1% (=1 g/l)   0.994, assuming all pairs of Cys residues form cystines


Ext. coefficient    53860
Abs 0.1% (=1 g/l)   0.985, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.03
This classifies the protein as unstable.



Aliphatic index: 80.70

Grand average of hydropathicity (GRAVY): -0.738