ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSKGKVCLAY SGGLDTSVIL AWLLDQGYEV VAFMANVGQE EDFDAAKEKA LKIGACKFVC 

        70         80         90        100        110        120 
VDCREDFVKD ILFPAVQVNA VYEDVYLLGT SLARPVIAKA QIDVAKQEGC FAVSHGCTGK 

       130        140        150        160        170        180 
GNDQIRFELS FYALKPDVKC ITPWRMPEFF ERFAGRKDLL DYAAQKGIPV AQTKAKPWST 

       190        200        210        220        230        240 
DENQAHISYE AGILEDPDTT PPKDMWKLIV DPMDAPDQPQ DLTIDFERGL PVKLTYTDNK 

       250        260        270        280        290        300 
TSKEVSVTKP LDVFLAASNL ARANGVGRID IVEDRYINLK SRGCYEQAPL TVLRKAHVDL 

       310        320        330        340        350        360 
EGLTLDKEVR QLRDSFVTPN YSRLIYNGFL LHPECEYIRS MIQPSQNSVN GTVRVRLYKG 

       370        380        390        400        410        420 
NVIILGRSTK TEKLYDPTES SMDELTGFLP TDTTGFIAIQ AIRIKKYGES KKTKGEELTL 

References and documentation are available.

Number of amino acids: 420

Molecular weight: 46939.77

Theoretical pI: 5.53

Amino acid composition: 
Ala (A)  32	  7.6%
Arg (R)  20	  4.8%
Asn (N)  13	  3.1%
Asp (D)  32	  7.6%
Cys (C)   9	  2.1%
Gln (Q)  16	  3.8%
Glu (E)  27	  6.4%
Gly (G)  27	  6.4%
His (H)   4	  1.0%
Ile (I)  24	  5.7%
Leu (L)  39	  9.3%
Lys (K)  33	  7.9%
Met (M)   7	  1.7%
Phe (F)  17	  4.0%
Pro (P)  21	  5.0%
Ser (S)  21	  5.0%
Thr (T)  26	  6.2%
Trp (W)   4	  1.0%
Tyr (Y)  16	  3.8%
Val (V)  32	  7.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      2100
Hydrogen    H	      3322
Nitrogen    N	       554
Oxygen      O	       631
Sulfur      S	        16

Formula: C2100H3322N554O631S16
Total number of atoms: 6623

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    46340
Abs 0.1% (=1 g/l)   0.987, assuming all pairs of Cys residues form cystines


Ext. coefficient    45840
Abs 0.1% (=1 g/l)   0.977, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 27.87
This classifies the protein as stable.



Aliphatic index: 88.21

Grand average of hydropathicity (GRAVY): -0.266