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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSKHHPDLIM CKKQTGIAIG RVCDKCDGKC VICDSYVRPT TLVRICDECN YGSFQGKCVI

70 80 90 100 110
CGGQGVSDAY YCFACSREEK DRDGCPKIVN LGGSKIDLFY ERKKYGFKKR


References and documentation are available.

Number of amino acids: 110 Molecular weight: 12351.38 Theoretical pI: 8.83
Amino acid composition: 
Ala (A) 3 2.7% Arg (R) 7 6.4% Asn (N) 2 1.8% Asp (D) 9 8.2% Cys (C) 12 10.9% Gln (Q) 3 2.7% Glu (E) 4 3.6% Gly (G) 12 10.9% His (H) 2 1.8% Ile (I) 8 7.3% Leu (L) 4 3.6% Lys (K) 13 11.8% Met (M) 2 1.8% Phe (F) 4 3.6% Pro (P) 3 2.7% Ser (S) 6 5.5% Thr (T) 3 2.7% Trp (W) 0 0.0% Tyr (Y) 6 5.5% Val (V) 7 6.4% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 13 Total number of positively charged residues (Arg + Lys): 20 Atomic composition: Carbon C 532 Hydrogen H 851 Nitrogen N 153 Oxygen O 157 Sulfur S 14 Formula: C532H851N153O157S14 Total number of atoms: 1707 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 9690 Abs 0.1% (=1 g/l) 0.785, assuming all pairs of Cys residues form cystines Ext. coefficient 8940 Abs 0.1% (=1 g/l) 0.724, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 37.54 This classifies the protein as stable. Aliphatic index: 63.73 Grand average of hydropathicity (GRAVY): -0.408