ProtParam
User-provided sequence:
10 20 30 40 50 60
MSKIEELPPS DTDDHSYSSK PGDVFLAFVD APVKETDDIL VEDSFIGGEP KWLHPDSEPP
70 80 90 100 110 120
AELLKCGACK SADNMKLLLQ AFSPLDDEQM SAIQQRLGIN NMSYINPQDD RVLYVFLCTE
130 140 150 160 170 180
CQRKGNSVRC IRGVKKNKNV DSLSEKMAST SLEKDFQINP FDLSNNSDSK CNAFSSNPFG
190 200 210 220 230 240
GANANPFGAD SINSNISQSK DEGKKKESAT VSAKTARKLH DLQKDKEYDG NKCFKSCLLY
250 260 270 280 290 300
VEEETFKNKK PAHLQLPKNL KIDKEALDLT GDEDLEKDPI KLDPRTEKLS KFLDDDTFQK
310 320 330 340 350 360
FQEVVGYNPL QVLRYDLGGK PLLYAETKVD ILSTVPRPGY NPSSQRIFEM QLMPKMIFDL
370 380 390 400
EEVVSVDNGM EWGTILVFTD VENYMPEFDE HGVGYVEECV KVQWESRT
References and
documentation are available.
Number of amino acids: 408
Molecular weight: 45993.80
Theoretical pI: 4.79
Total number of negatively charged residues (Asp + Glu): 71
Total number of positively charged residues (Arg + Lys): 50
Atomic composition:
Carbon C 2026
Hydrogen H 3174
Nitrogen N 532
Oxygen O 650
Sulfur S 19
Formula: C
2026H
3174N
532O
650S
19
Total number of atoms: 6401
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 33390
Abs 0.1% (=1 g/l) 0.726, assuming all pairs of Cys residues form cystines
Ext. coefficient 32890
Abs 0.1% (=1 g/l) 0.715, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.47
This classifies the protein as unstable.
Aliphatic index: 73.80
Grand average of hydropathicity (GRAVY): -0.626