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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSKIEELPPS DTDDHSYSSK PGDVFLAFVD APVKETDDIL VEDSFIGGEP KWLHPDSEPP

70 80 90 100 110 120
AELLKCGACK SADNMKLLLQ AFSPLDDEQM SAIQQRLGIN NMSYINPQDD RVLYVFLCTE

130 140 150 160 170 180
CQRKGNSVRC IRGVKKNKNV DSLSEKMAST SLEKDFQINP FDLSNNSDSK CNAFSSNPFG

190 200 210 220 230 240
GANANPFGAD SINSNISQSK DEGKKKESAT VSAKTARKLH DLQKDKEYDG NKCFKSCLLY

250 260 270 280 290 300
VEEETFKNKK PAHLQLPKNL KIDKEALDLT GDEDLEKDPI KLDPRTEKLS KFLDDDTFQK

310 320 330 340 350 360
FQEVVGYNPL QVLRYDLGGK PLLYAETKVD ILSTVPRPGY NPSSQRIFEM QLMPKMIFDL

370 380 390 400
EEVVSVDNGM EWGTILVFTD VENYMPEFDE HGVGYVEECV KVQWESRT


References and documentation are available.

Number of amino acids: 408 Molecular weight: 45993.80 Theoretical pI: 4.79
Amino acid composition: 
Ala (A) 18 4.4% Arg (R) 11 2.7% Asn (N) 23 5.6% Asp (D) 38 9.3% Cys (C) 9 2.2% Gln (Q) 16 3.9% Glu (E) 33 8.1% Gly (G) 21 5.1% His (H) 5 1.2% Ile (I) 16 3.9% Leu (L) 38 9.3% Lys (K) 39 9.6% Met (M) 10 2.5% Phe (F) 19 4.7% Pro (P) 24 5.9% Ser (S) 34 8.3% Thr (T) 15 3.7% Trp (W) 3 0.7% Tyr (Y) 11 2.7% Val (V) 25 6.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 71 Total number of positively charged residues (Arg + Lys): 50 Atomic composition: Carbon C 2026 Hydrogen H 3174 Nitrogen N 532 Oxygen O 650 Sulfur S 19 Formula: C2026H3174N532O650S19 Total number of atoms: 6401 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 33390 Abs 0.1% (=1 g/l) 0.726, assuming all pairs of Cys residues form cystines Ext. coefficient 32890 Abs 0.1% (=1 g/l) 0.715, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 48.47 This classifies the protein as unstable. Aliphatic index: 73.80 Grand average of hydropathicity (GRAVY): -0.626