ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSKIKVVHPI VEMDGDEQTR VIWKLIKEKL ILPYLDVDLK YYDLSIQERD RTNDQVTKDS 

        70         80         90        100        110        120 
SYATLKYGVA VKCATITPDE ARMKEFNLKE MWKSPNGTIR NILGGTVFRE PIIIPKIPRL 

       130        140        150        160        170        180 
VPHWEKPIII GRHAFGDQYR ATDIKIKKAG KLRLQFSSDD GKENIDLKVY EFPKSGGIAM 

       190        200        210        220        230        240 
AMFNTNDSIK GFAKASFELA LKRKLPLFFT TKNTILKNYD NQFKQIFDNL FDKEYKEKFQ 

       250        260        270        280        290        300 
ALKITYEHRL IDDMVAQMLK SKGGFIIAMK NYDGDVQSDI VAQGFGSLGL MTSILITPDG 

       310        320        330        340        350        360 
KTFESEAAHG TVTRHFRKHQ RGEETSTNSI ASIFAWTRAI IQRGKLDNTD DVIKFGNLLE 

       370        380        390        400        410        420 
KATLDTVQVG GKMTKDLALM LGKTNRSSYV TTEEFIDEVA KRLQNMMLSS NEDKKGMCKL 

References and documentation are available.

Number of amino acids: 420

Molecular weight: 47856.37

Theoretical pI: 9.24

Amino acid composition: 
Ala (A)  24	  5.7%
Arg (R)  19	  4.5%
Asn (N)  18	  4.3%
Asp (D)  31	  7.4%
Cys (C)   2	  0.5%
Gln (Q)  15	  3.6%
Glu (E)  24	  5.7%
Gly (G)  27	  6.4%
His (H)   7	  1.7%
Ile (I)  36	  8.6%
Leu (L)  35	  8.3%
Lys (K)  47	 11.2%
Met (M)  15	  3.6%
Phe (F)  21	  5.0%
Pro (P)  12	  2.9%
Ser (S)  22	  5.2%
Thr (T)  29	  6.9%
Trp (W)   4	  1.0%
Tyr (Y)  12	  2.9%
Val (V)  20	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 66

Atomic composition:

Carbon      C	      2145
Hydrogen    H	      3435
Nitrogen    N	       575
Oxygen      O	       627
Sulfur      S	        17

Formula: C2145H3435N575O627S17
Total number of atoms: 6799

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40005
Abs 0.1% (=1 g/l)   0.836, assuming all pairs of Cys residues form cystines


Ext. coefficient    39880
Abs 0.1% (=1 g/l)   0.833, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 19.79
This classifies the protein as stable.



Aliphatic index: 85.45

Grand average of hydropathicity (GRAVY): -0.409