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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSKIVKRKEK KANDELTSLA EKIRAKALEN QKKLIEAEKE GGSESDSEED ATAEKKKVLK

70 80 90 100 110 120
SKSKSTVSTQ NENTNEDESF ESFSELNLVP ELIQACKNLN YSKPTPIQSK AIPPALEGHD

130 140 150 160 170 180
IIGLAQTGSG KTAAFAIPIL NRLWHDQEPY YACILAPTRE LAQQIKETFD SLGSLMGVRS

190 200 210 220 230 240
TCIVGGMNMM DQARDLMRKP HIIIATPGRL MDHLENTKGF SLRKLKFLVM DEADRLLDME

250 260 270 280 290 300
FGPVLDRILK IIPTQERTTY LFSATMTSKI DKLQRASLTN PVKCAVSNKY QTVDTLVQTL

310 320 330 340 350 360
MVVPGGLKNT YLIYLLNEFI GKTMIIFTRT KANAERLSGL CNLLEFSATA LHGDLNQNQR

370 380 390 400 410 420
MGSLDLFKAG KRSILVATDV AARGLDIPSV DIVVNYDIPV DSKSYIHRVG RTARAGRSGK

430 440 450 460 470 480
SISLVSQYDL ELILRIEEVL GKKLPKESVD KNIILTLRDS VDKANGEVVM EMNRRNKEKI

490 500
ARGKGRRGRM MTRENMDMGE R


References and documentation are available.

Number of amino acids: 501 Molecular weight: 55968.72 Theoretical pI: 9.22
Amino acid composition: 
Ala (A) 35 7.0% Arg (R) 32 6.4% Asn (N) 25 5.0% Asp (D) 28 5.6% Cys (C) 5 1.0% Gln (Q) 16 3.2% Glu (E) 37 7.4% Gly (G) 30 6.0% His (H) 6 1.2% Ile (I) 35 7.0% Leu (L) 54 10.8% Lys (K) 45 9.0% Met (M) 19 3.8% Phe (F) 12 2.4% Pro (P) 17 3.4% Ser (S) 36 7.2% Thr (T) 31 6.2% Trp (W) 1 0.2% Tyr (Y) 10 2.0% Val (V) 27 5.4% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 65 Total number of positively charged residues (Arg + Lys): 77 Atomic composition: Carbon C 2445 Hydrogen H 4052 Nitrogen N 696 Oxygen O 750 Sulfur S 24 Formula: C2445H4052N696O750S24 Total number of atoms: 7967 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 20650 Abs 0.1% (=1 g/l) 0.369, assuming all pairs of Cys residues form cystines Ext. coefficient 20400 Abs 0.1% (=1 g/l) 0.364, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 32.56 This classifies the protein as stable. Aliphatic index: 91.90 Grand average of hydropathicity (GRAVY): -0.383