ProtParam
User-provided sequence:
10 20 30 40 50 60
MSKIVKRKEK KANDELTSLA EKIRAKALEN QKKLIEAEKE GGSESDSEED ATAEKKKVLK
70 80 90 100 110 120
SKSKSTVSTQ NENTNEDESF ESFSELNLVP ELIQACKNLN YSKPTPIQSK AIPPALEGHD
130 140 150 160 170 180
IIGLAQTGSG KTAAFAIPIL NRLWHDQEPY YACILAPTRE LAQQIKETFD SLGSLMGVRS
190 200 210 220 230 240
TCIVGGMNMM DQARDLMRKP HIIIATPGRL MDHLENTKGF SLRKLKFLVM DEADRLLDME
250 260 270 280 290 300
FGPVLDRILK IIPTQERTTY LFSATMTSKI DKLQRASLTN PVKCAVSNKY QTVDTLVQTL
310 320 330 340 350 360
MVVPGGLKNT YLIYLLNEFI GKTMIIFTRT KANAERLSGL CNLLEFSATA LHGDLNQNQR
370 380 390 400 410 420
MGSLDLFKAG KRSILVATDV AARGLDIPSV DIVVNYDIPV DSKSYIHRVG RTARAGRSGK
430 440 450 460 470 480
SISLVSQYDL ELILRIEEVL GKKLPKESVD KNIILTLRDS VDKANGEVVM EMNRRNKEKI
490 500
ARGKGRRGRM MTRENMDMGE R
References and
documentation are available.
Number of amino acids: 501
Molecular weight: 55968.72
Theoretical pI: 9.22
Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 77
Atomic composition:
Carbon C 2445
Hydrogen H 4052
Nitrogen N 696
Oxygen O 750
Sulfur S 24
Formula: C
2445H
4052N
696O
750S
24
Total number of atoms: 7967
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 20650
Abs 0.1% (=1 g/l) 0.369, assuming all pairs of Cys residues form cystines
Ext. coefficient 20400
Abs 0.1% (=1 g/l) 0.364, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.56
This classifies the protein as stable.
Aliphatic index: 91.90
Grand average of hydropathicity (GRAVY): -0.383