ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSKKFSSKNS QRYVVVHRPH DDPSFYDTDA SAHVLVPVSN PNKTSPEADL RKKDVSSTKP 

        70         80         90        100        110        120 
KGRRAHVGEA ALYGINFDDS EYDYTQHLKP IGLDPENSIF IASKGNEQKV EKKNIEDLFI 

       130        140        150        160        170        180 
EPKYRRDEIE KDDALPVFQR GMAKPEYLLH QQDTTDEIRG FKPDMNPALR EVLEALEDEA 

       190        200        210        220        230        240 
YVVNDDVVVE DISKKTQLQG DNYGEEEKED DIFAQLLGSG EAKDEDEFED EFDEWDIDNV 

       250        260        270        280        290        300 
ENFEDENYVK EMAQFDNIEN LEDLENIDYQ ADVRRFQKDN SILEKHNSDD EFSNAGLDSV 

       310        320        330        340        350        360 
NPSEEEDVLG ELPSIQDKSK TGKKKRKSRQ KKGAMSDVSG FSMSSSAIAR TETMTVLDDQ 

       370        380        390        400        410        420 
YDQIINGYEN YEEELEEDEE QNYQPFDMSA ERSDFESMLD DFLDNYELES GGRKLAKKDK 

       430        440        450        460 
EIERLKEAAD EVSKGKLSQR RNRERQEKKK LEKVTNTLSS LKF 

References and documentation are available.

Number of amino acids: 463

Molecular weight: 53390.35

Theoretical pI: 4.53

Amino acid composition: 
Ala (A)  24	  5.2%
Arg (R)  22	  4.8%
Asn (N)  26	  5.6%
Asp (D)  53	 11.4%
Cys (C)   0	  0.0%
Gln (Q)  20	  4.3%
Glu (E)  60	 13.0%
Gly (G)  20	  4.3%
His (H)   7	  1.5%
Ile (I)  19	  4.1%
Leu (L)  32	  6.9%
Lys (K)  44	  9.5%
Met (M)   9	  1.9%
Phe (F)  19	  4.1%
Pro (P)  16	  3.5%
Ser (S)  37	  8.0%
Thr (T)  13	  2.8%
Trp (W)   1	  0.2%
Tyr (Y)  16	  3.5%
Val (V)  25	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 113
Total number of positively charged residues (Arg + Lys): 66

Atomic composition:

Carbon      C	      2311
Hydrogen    H	      3604
Nitrogen    N	       634
Oxygen      O	       802
Sulfur      S	         9

Formula: C2311H3604N634O802S9
Total number of atoms: 7360

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    29340
Abs 0.1% (=1 g/l)   0.550

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.80
This classifies the protein as unstable.



Aliphatic index: 63.80

Grand average of hydropathicity (GRAVY): -1.119