ProtParam
User-provided sequence:
10 20 30 40 50 60
MSKKLASPLS FLPLYNLLSA VGWSYLLYLV ISLYPKVGQP AFFYQTKNVA TLVQCGAIIE
70 80 90 100 110 120
IINSFLGVVR SPLLTTVAQV SSRLLVVLGI FQLLPNTSGV QSVVYISLLL AWSITEIVRY
130 140 150 160 170 180
LYYFFMLVFK NGAPKILILL RYNLFWILYP TGVASELRII YCALNAAESQ YSLLYKRILI
190 200 210
AAMLAYIPGF PMLFLHMVAQ RKKVMKSLRS SFGKKLI
References and
documentation are available.
Number of amino acids: 217
Molecular weight: 24510.66
Theoretical pI: 9.98
Total number of negatively charged residues (Asp + Glu): 4
Total number of positively charged residues (Arg + Lys): 20
Atomic composition:
Carbon C 1169
Hydrogen H 1847
Nitrogen N 273
Oxygen O 283
Sulfur S 8
Formula: C
1169H
1847N
273O
283S
8
Total number of atoms: 3580
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 38975
Abs 0.1% (=1 g/l) 1.590, assuming all pairs of Cys residues form cystines
Ext. coefficient 38850
Abs 0.1% (=1 g/l) 1.585, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.51
This classifies the protein as stable.
Aliphatic index: 135.21
Grand average of hydropathicity (GRAVY): 0.794