ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSKKLSLEER LSLATKKGRK KNKRSTSNLS SPSPVVLSNN EQESARTSID DAAAGVVSID 

        70         80         90        100        110        120 
NAENIDDPAV RSESTVEGDT GKADSIAVDD VVHPDHNRTD CFDDTMVSLP TWLPKNYTEF 

       130        140        150        160        170        180 
TVEELVKEIS PEYLRLNKQI DDLTNELNRK SQIETTDSSF FKLIKEKDDL IDQLRKEGAK 

       190        200        210        220        230        240 
LAETELRQSN QIKALRTKVK DLEYEVSELN DSSAQSVENY NELQSLYHNI QGQLAEATNK 

       250        260        270        280        290        300 
LKDADKQKES LETLEKNIKE KDDLITILQQ SLDNMRTLLE KEKSEFQTEK KALQEATVDQ 

       310        320        330        340        350        360 
VTTLETKLEQ LRIELDSSTQ NLDAKSNRDF VDDQQSYEEK QHASFQYNRL KEQLESSKAN 

       370        380        390        400        410        420 
WDSIEYALNT KIVNLENRFE STMKEKNDIE EKYQTALRSS ETLGKQLEKE KENHSKAVLE 

       430        440        450        460        470        480 
VKDLERRAET LKSSLQSISD DYNLLKKKYE IQRSQLEQKE NELKPHQENS NEKIIDKIPV 

       490        500        510        520        530        540 
ELTDSLNSME GNIEDEWTLP QENSMLSLSM SKLGELESDP SLKPIYNESH ETICSEESQH 

       550        560        570        580        590        600 
FDRKNVDFSI DDIPEEAAAL QAIREGESMN SLNNTSIPYR RASVQLSNSN GHISAHLVNK 

       610        620        630        640        650        660 
LSTELKRLEG ELSASKELYD NLLKEKTKAN DEILRLLEEN DKFNEVNKQK DDLLKRVEQM 

       670        680        690        700 
QSKLETSLQL LGEKTEQVEE LENDVSDLKE MMHQQVQQMV EMQGKMR 

References and documentation are available.

Number of amino acids: 707

Molecular weight: 81171.12

Theoretical pI: 4.81

Amino acid composition: 
Ala (A)  33	  4.7%
Arg (R)  28	  4.0%
Asn (N)  49	  6.9%
Asp (D)  52	  7.4%
Cys (C)   2	  0.3%
Gln (Q)  44	  6.2%
Glu (E)  92	 13.0%
Gly (G)  14	  2.0%
His (H)  11	  1.6%
Ile (I)  32	  4.5%
Leu (L)  83	 11.7%
Lys (K)  70	  9.9%
Met (M)  14	  2.0%
Phe (F)  11	  1.6%
Pro (P)  14	  2.0%
Ser (S)  72	 10.2%
Thr (T)  38	  5.4%
Trp (W)   3	  0.4%
Tyr (Y)  14	  2.0%
Val (V)  31	  4.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 144
Total number of positively charged residues (Arg + Lys): 98

Atomic composition:

Carbon      C	      3482
Hydrogen    H	      5671
Nitrogen    N	       979
Oxygen      O	      1213
Sulfur      S	        16

Formula: C3482H5671N979O1213S16
Total number of atoms: 11361

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    37485
Abs 0.1% (=1 g/l)   0.462, assuming all pairs of Cys residues form cystines


Ext. coefficient    37360
Abs 0.1% (=1 g/l)   0.460, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.13
This classifies the protein as unstable.



Aliphatic index: 80.82

Grand average of hydropathicity (GRAVY): -0.969