ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSKKPKSVHF STSSPKSFLS SFPSFTSLPA SPLNQTFSQS MMEETVEAAE SIIKKWDPNS 

        70         80         90        100        110        120 
PSYTKIISLF SHSRREAKEF IRCIRDLRRA MHFLISQHSK SAKLVLAQHL MQIAMARLEK 

       130        140        150        160        170        180 
EFFQILSSNR DQLDPESVSG HSSISSNSEF EDVMQSSDDE EEDELKKAGE TITKVEKAAA 

       190        200        210        220        230        240 
LVMSDLKVIA ETMISCGYGK ECIKSYKLIR KSIVDEGLHL LGIEKCKISR FNRMDWDVLE 

       250        260        270        280        290        300 
HMIKNWIKAA KIGVITLLRG EKLLCDHVFS ASSTIRESCF YEIVNEAGIN LFKFPELVAE 

       310        320        330        340        350        360 
KKPSPERIFR LMDLYAAISD LRPDIELIFH FDSVAAVKTL VLSSLKKLKD SIYTSLMEFE 

       370        380        390        400        410        420 
STIQKDSSKA LTAGGGIHKL TRSTMSFISS LSEYSRVLSE ILAEHPLKKN TRMLESYFTA 

       430        440        450        460        470        480 
PILEDEHNNH AVSVHLAWLI LIFLCKLDIK AESYKDVSLS YLFLVNNIQF VVDTVRSTHL 

       490        500        510        520        530        540 
RNLLGDDWLT KHEAKLRSYA ANYEIAAWAN VYISLPEKTS SRLSPEEAKT HFKRFHAVFE 

       550        560        570        580        590        600 
EAYMKQSSCV ITDAKLRNEL KVSIAKKIVP EYREFYGKYL PTLSKERNIE MLVSFKPDNL 

       610        620        630 
ENYLSDLFHG TPILSGSSSS SSSLSSSSCI SLGCVRN 

References and documentation are available.

Number of amino acids: 637

Molecular weight: 72180.05

Theoretical pI: 7.92

Amino acid composition: 
Ala (A)  41	  6.4%
Arg (R)  28	  4.4%
Asn (N)  21	  3.3%
Asp (D)  28	  4.4%
Cys (C)  10	  1.6%
Gln (Q)  11	  1.7%
Glu (E)  52	  8.2%
Gly (G)  17	  2.7%
His (H)  20	  3.1%
Ile (I)  47	  7.4%
Leu (L)  69	 10.8%
Lys (K)  54	  8.5%
Met (M)  17	  2.7%
Phe (F)  31	  4.9%
Pro (P)  20	  3.1%
Ser (S)  89	 14.0%
Thr (T)  26	  4.1%
Trp (W)   6	  0.9%
Tyr (Y)  18	  2.8%
Val (V)  32	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 80
Total number of positively charged residues (Arg + Lys): 82

Atomic composition:

Carbon      C	      3229
Hydrogen    H	      5135
Nitrogen    N	       853
Oxygen      O	       963
Sulfur      S	        27

Formula: C3229H5135N853O963S27
Total number of atoms: 10207

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    60445
Abs 0.1% (=1 g/l)   0.837, assuming all pairs of Cys residues form cystines


Ext. coefficient    59820
Abs 0.1% (=1 g/l)   0.829, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.12
This classifies the protein as unstable.



Aliphatic index: 92.03

Grand average of hydropathicity (GRAVY): -0.194