ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSKKSTESDA GNTEKQWSLA DFEIGRPLGK GKFGRVYLAR EAKSKYIVAL KVIFKEQIEK 

        70         80         90        100        110        120 
YKIHHQLRRE MEIQTSLRHP NILRLFGWFH DNERIFLILE YAHGGELYGV LKQNGHLTEQ 

       130        140        150        160        170        180 
QAATYIASLS QALAYCHGKC VIHRDIKPEN LLLDHEGRLK IADFGWSVQS SNKRKTMCGT 

       190        200        210        220        230        240 
LDYLAPEMVE NRDHDYAVDN WTLGILCYEF LYGNPPFEAE SQKDTFKRIL KIDLSFPLTP 

       250        260        270        280 
NVSEEAKNLI SQLLVKDPSK RLSIEKIMQH PWIVKNADPK GVCASIDI 

References and documentation are available.

Number of amino acids: 288

Molecular weight: 33119.09

Theoretical pI: 8.44

Amino acid composition: 
Ala (A)  18	  6.2%
Arg (R)  14	  4.9%
Asn (N)  12	  4.2%
Asp (D)  15	  5.2%
Cys (C)   5	  1.7%
Gln (Q)  12	  4.2%
Glu (E)  22	  7.6%
Gly (G)  17	  5.9%
His (H)  11	  3.8%
Ile (I)  22	  7.6%
Leu (L)  31	 10.8%
Lys (K)  26	  9.0%
Met (M)   5	  1.7%
Phe (F)  11	  3.8%
Pro (P)  11	  3.8%
Ser (S)  18	  6.2%
Thr (T)  10	  3.5%
Trp (W)   5	  1.7%
Tyr (Y)  11	  3.8%
Val (V)  12	  4.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 40

Atomic composition:

Carbon      C	      1492
Hydrogen    H	      2343
Nitrogen    N	       407
Oxygen      O	       426
Sulfur      S	        10

Formula: C1492H2343N407O426S10
Total number of atoms: 4678

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    44140
Abs 0.1% (=1 g/l)   1.333, assuming all pairs of Cys residues form cystines


Ext. coefficient    43890
Abs 0.1% (=1 g/l)   1.325, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.36
This classifies the protein as stable.



Aliphatic index: 90.10

Grand average of hydropathicity (GRAVY): -0.435