ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSKKVQAKQG TDDLVMLPKV SEDEICENLK KRYMNDFIYT NIGPVLISVN PFRNLNNSGP 

        70         80         90        100        110        120 
DFIEAYRGKH AQEVPPHVYQ LAESAYRAMK NDQENQCVII SGESGAGKTE AAKLIMGYVS 

       130        140        150        160        170        180 
AISGSTEKVE YVKHVILESN PLLEAFGNAK TLRNNNSSRF GKYFEIQFDK AGDPVGGKIY 

       190        200        210        220        230        240 
NYLLEKSRVV YQNPGERNFH IFYQLLAGAS AQEKRDYVLS SPESYYYLNQ SQCYTVDGIN 

       250        260        270        280        290        300 
DVSDYAEVRQ AMDTIGLTAQ EQSDIIRIVA CVLHIGNIYF IEDDKGNAAI YDPNALELAA 

       310        320        330        340        350        360 
SMLCIDSATL QNAILFRVIN TGGAGGAGNR RSTYNVPQNV EQANGTRDAL ARTIYDRMFS 

       370        380        390        400        410        420 
WLVERVNQSL SYYKSPYQNV IGILDIFGFE IFEKNGFEQF CINFVNEKLQ QFFIELTLKA 

       430        440        450        460        470        480 
EQEEYVREGI KWEPIKYFNN QIVCDLIEGK SPPGIFSLLD DICSTLHAQS TGTDQKFLEK 

       490        500        510        520        530        540 
MAGIYDGHLH WRGMTGAFAI KHYAGEVTYE AEGFSDKNKD TLFFDLIEAI QCSKMPFLAS 

       550        560        570        580        590        600 
LFNEDTGSLQ KKRPTTAGFK IKTSAGELMK ALSQCTPHYI RCIKPNETKK AKDWENSRVK 

       610        620        630        640        650        660 
HQVQYLGLLE NVRVRRAGFA YRNTFDKVLK RYKKLSSKTW GIWGEWKGDA IEGCKTIFQD 

       670        680        690        700        710        720 
MNLEAGQWQL GKTKVFIRHP ETVFLLEEAL DKKDFDCTAK IQKAFRNWKA KKHSLEQRAQ 

       730        740        750        760        770        780 
IAHMFKDKKE RQRNSIDRKF TSDYIDFENQ FGLQEAMQNA HKKERVVFAD TVIKIDRRAK 

       790        800        810        820        830        840 
QKNYEMVLTD QALYFVEKSI KKKVLVHTLI RRVGLREIKG VSISTLSDNV IVFHLPEHDQ 

       850        860        870        880        890        900 
VIENDKKTEI IIVLVEYFKA IGGGSLNVQF SDRINYTLKK GEQKEISFQK SEQCPTLVVK 

       910        920        930        940        950        960 
KGGKGLIGTI ASGLPSSTDS TPKNYNPNSM SQASSRPAPQ QSAGRGRGMP QGAGQPQPQQ 

       970        980        990       1000       1010       1020 
PQQQQRPMPQ PQQGGGARPM PQPQQGGGAR PMGAPQQGGA PQQGAGRQLP QPTQQGGAPG 

      1030       1040       1050       1060       1070       1080 
GRGAPMGRGA PGGGPAGAGG RPLPTVAKPA PQPSRPTAKA LYDYDASSTD ELSFKEGDII 

      1090       1100       1110 
FIVQKDNGGW TQGELKSGQK GWAPTNYLQY N 

References and documentation are available.

Number of amino acids: 1111

Molecular weight: 124256.95

Theoretical pI: 8.93

Amino acid composition: 
Ala (A)  82	  7.4%
Arg (R)  52	  4.7%
Asn (N)  57	  5.1%
Asp (D)  54	  4.9%
Cys (C)  14	  1.3%
Gln (Q)  80	  7.2%
Glu (E)  69	  6.2%
Gly (G)  97	  8.7%
His (H)  18	  1.6%
Ile (I)  72	  6.5%
Leu (L)  75	  6.8%
Lys (K)  89	  8.0%
Met (M)  22	  2.0%
Phe (F)  49	  4.4%
Pro (P)  54	  4.9%
Ser (S)  66	  5.9%
Thr (T)  49	  4.4%
Trp (W)  11	  1.0%
Tyr (Y)  44	  4.0%
Val (V)  57	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 123
Total number of positively charged residues (Arg + Lys): 141

Atomic composition:

Carbon      C	      5524
Hydrogen    H	      8652
Nitrogen    N	      1540
Oxygen      O	      1654
Sulfur      S	        36

Formula: C5524H8652N1540O1654S36
Total number of atoms: 17406

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   126935
Abs 0.1% (=1 g/l)   1.022, assuming all pairs of Cys residues form cystines


Ext. coefficient   126060
Abs 0.1% (=1 g/l)   1.015, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.75
This classifies the protein as unstable.



Aliphatic index: 73.86

Grand average of hydropathicity (GRAVY): -0.556