ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSKLGQEKNE VNYSDVREDR VVTNSTGNPI NEPFVTQRIG EHGPLLLQDY NLIDSLAHFN 

        70         80         90        100        110        120 
RENIPQRNPH AHGSGAFGYF EVTDDITDIC GSAMFSKIGK RTKCLTRFST VGGDKGSADT 

       130        140        150        160        170        180 
VRDPRGFATK FYTEEGNLDW VYNNTPVFFI RDPSKFPHFI HTQKRNPQTN LRDADMFWDF 

       190        200        210        220        230        240 
LTTPENQVAI HQVMILFSDR GTPANYRSMH GYSGHTYKWS NKNGDWHYVQ VHIKTDQGIK 

       250        260        270        280        290        300 
NLTIEEATKI AGSNPDYCQQ DLFEAIQNGN YPSWTVYIQT MTERDAKKLP FSVFDLTKVW 

       310        320        330        340        350        360 
PQGQFPLRRV GKIVLNENPL NFFAQVEQAA FAPSTTVPYQ EASADPVLQA RLFSYADAHR 

       370        380        390        400        410        420 
YRLGPNFHQI PVNCPYASKF FNPAIRDGPM NVNGNFGSEP TYLANDKSYT YIQQDRPIQQ 

       430        440        450        460        470        480 
HQEVWNGPAI PYHWATSPGD VDFVQARNLY RVLGKQPGQQ KNLAYNIGIH VEGACPQIQQ 

       490        500        510 
RVYDMFARVD KGLSEAIKKV AEAKHASELS SNSKF 

References and documentation are available.

Number of amino acids: 515

Molecular weight: 58555.48

Theoretical pI: 7.00

Amino acid composition: 
Ala (A)  35	  6.8%
Arg (R)  26	  5.0%
Asn (N)  37	  7.2%
Asp (D)  31	  6.0%
Cys (C)   5	  1.0%
Gln (Q)  33	  6.4%
Glu (E)  23	  4.5%
Gly (G)  34	  6.6%
His (H)  17	  3.3%
Ile (I)  27	  5.2%
Leu (L)  27	  5.2%
Lys (K)  27	  5.2%
Met (M)   8	  1.6%
Phe (F)  30	  5.8%
Pro (P)  33	  6.4%
Ser (S)  29	  5.6%
Thr (T)  30	  5.8%
Trp (W)   8	  1.6%
Tyr (Y)  23	  4.5%
Val (V)  32	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 54
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      2621
Hydrogen    H	      3958
Nitrogen    N	       732
Oxygen      O	       776
Sulfur      S	        13

Formula: C2621H3958N732O776S13
Total number of atoms: 8100

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    78520
Abs 0.1% (=1 g/l)   1.341, assuming all pairs of Cys residues form cystines


Ext. coefficient    78270
Abs 0.1% (=1 g/l)   1.337, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 30.26
This classifies the protein as stable.



Aliphatic index: 65.71

Grand average of hydropathicity (GRAVY): -0.631