ProtParam
User-provided sequence:
10 20 30 40 50 60
MSKLLKLVIV GDGGVGKSAL TIQLTQNQFI AEYDPTIENS YRKQVNIDEE VYMLDILDTA
70 80 90 100 110 120
GQEEYSAMRD QYIRSGRGFL IVYSIISRAS FEAVTTFREQ ILRVKDLSTY PIVIIGNKAD
130 140 150 160 170 180
LPDKDRKVPP MEGKELAKSF GAPFLETSAK SRVNVEEAFF TLVREIKRWN QNPQNEEMLP
PKKRGCIIL
References and
documentation are available.
Number of amino acids: 189
Molecular weight: 21495.76
Theoretical pI: 6.42
Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 26
Atomic composition:
Carbon C 962
Hydrogen H 1545
Nitrogen N 257
Oxygen O 287
Sulfur S 6
Formula: C
962H
1545N
257O
287S
6
Total number of atoms: 3057
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 15930
Abs 0.1% (=1 g/l) 0.741, assuming all pairs of Cys residues form cystines
Ext. coefficient 15930
Abs 0.1% (=1 g/l) 0.741, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.33
This classifies the protein as unstable.
Aliphatic index: 93.86
Grand average of hydropathicity (GRAVY): -0.305