ProtParam
User-provided sequence:
10 20 30 40 50 60
MSKLSKDYVS DSDSDDEVIS NEFSIPDGFK KCKHLKNFPL NGDNKKKAKQ QQVWLIKFPS
70 80 90 100 110 120
NVDISKLKSL PVDFESSTTM TIDKHDYKIM DDTDIESSLT QDNLSNMTLL VPSESKESLK
130 140 150 160 170 180
IASTAKDNAP LQFDKVFSVS ETAKIPAIDY SKVRVPRKDV PKVEGLKLEH FATGYDAEDF
190 200 210 220 230
HVAEEVKENK KEPKKRSHHD DEEESSEKKK KKKEKREKRE KKDKKDKKKK HRD
References and
documentation are available.
Number of amino acids: 233
Molecular weight: 26875.36
Theoretical pI: 8.76
Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 51
Atomic composition:
Carbon C 1179
Hydrogen H 1907
Nitrogen N 325
Oxygen O 380
Sulfur S 5
Formula: C
1179H
1907N
325O
380S
5
Total number of atoms: 3796
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 11460
Abs 0.1% (=1 g/l) 0.426, assuming all pairs of Cys residues form cystines
Ext. coefficient 11460
Abs 0.1% (=1 g/l) 0.426, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.69
This classifies the protein as stable.
Aliphatic index: 61.46
Grand average of hydropathicity (GRAVY): -1.201